RAPD标记在紫菜遗传多样性检测和种质鉴定中的应用 |
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引用本文: | 贾建航,王斌.RAPD标记在紫菜遗传多样性检测和种质鉴定中的应用[J].Acta Botanica Sinica,2000,42(4):403-407. |
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作者姓名: | 贾建航 王斌 |
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作者单位: | 中国科学院遗传研究所 |
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基金项目: | “8 63”OceanologyProjectofChinaHigh_TechProgram ( 819_0 3_0 2 ) |
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摘 要: | 用RAPD技术对4类紫菜(Porphyra yezoensis,P.haitanensis,P.katadni var.hemiphylla和P.oligospermatangia)的15个无性系丝状体进行了遗传多样履分析,从50个OPERON引物中经过初筛,其中6个引物可以扩增出稳定的可重复的图谱。这6个引物共扩增出了60条带,多态性比例达97.1%。根据RAPD结果将这15个无性了紫菜的DNA
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关 键 词: | RAPD 遗传多样性 聚类分析 紫菜 种质鉴定 |
The Application of RAPD Markers in Diversity Detection and Variety Identification of Porphyra |
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Authors: | JIA Jian_Hang WANG Ping JIN De_Min QU Xue_Ping WANG Qian LI Chuan_You WENG Man_Li WANG Bin [WTBZ] |
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Institution: | JIA Jian_Hang,WANG Ping,JIN De_Min,QU Xue_Ping,WANG Qian,LI Chuan_You,WENG Man_Li,WANG Bin * [WT6BZ] |
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Abstract: | RAPD (random amplified polymorphic DNA) markers were generated from filaments of 15 Porphyra lines representing four important groups, P. yezoensis, P. haitanensis, P. katadai var. hemiphylla and P. oligospermatangia . Among the total 69 fragments generated by 6 selected primers (among 50 primers), 67 appeared to be polymorphic (97.1%). Cluster analysis based on the RAPD results was performed. The 15 Porphyra lines were divided into 3 groups. This result was consistent with that from taxonomy analysis. A DNA fingerprinting based on 8 bands amplified with OPN_02 and OPJ_18 was constructed and might be used in Porphyra variety identification. Five specific RAPD fragments of 5 Porphyra lines were isolated and cloned into pGEM_T easy vector. These five RAPD fragments may be useful in germplasm identification and property protection of Porphyra . |
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Keywords: | RAPD genetic diversity cluster analysis Porphyra germplasm identification |
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