| 银杏性别相关分子标记 |
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| 引用本文： | 姜凌,尤瑞麟,李懋学,时晨.银杏性别相关分子标记[J].Acta Botanica Sinica,2003,45(6):742-747. |
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| 作者姓名： | 姜凌 尤瑞麟 李懋学 时晨 |
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| 作者单位： | 北京大学生命科学学院 北京100871
(姜凌，尤瑞麟，李懋学)，北京大学生命科学学院 北京100871(时晨) |
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| 基金项目： | 国家自然科学基金 (3 0 0 70 0 48)~~ |
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| 摘 要： | 利用RAPD技术寻找银杏(Ginkgo biloba L.)中与性别相关的分子标记。筛选了1200个10bp的随机引物，产生了8372个RAPD条带。只有S1478产生一条大小为682bp、雄性特异的分子标记，该分子标记被命名为S1478—682，出现在所有雄性植株中，而所有雌性植株都不具有该分子标记。通过在北京和沈阳种植的银杏植株的RAPD推广验证，说明该分子标记可以用来检测银杏植株的性别。
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| 关 键 词： | 银杏 性别决定 RAPD 分子标记 植株 |
Identification of a Sex-Associated RAPD Marker in Ginkgo biloba |
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| Authors: | JIANG Ling YOU Rui_Lin LI Mao_Xue SHI Chen |
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| Institution: | JIANG Ling,YOU Rui_Lin *,LI Mao_Xue,SHI Chen |
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| Abstract: | The random amplified polymorphic DNA (RAPD) technique was used to amplify DNA fragment, aiming at finding markers linked to the sex determination in Ginkgo biloba L. One thousand and two hundred random decamers had been screened. Of the 8 372 RAPD bands, only a 682 bp RAPD marker generated by a primer (S1478) of random decamer sequence, named S1478_682, was found to be associated with the male plants. This marker was present in all male plants and absent in all female plants. Ginkgo trees collected in both Beijing and Shenyang, China were tested using primer S1478. Positive results were obtained, suggesting S1478_682 could be utilized as a reliable RAPD marker to detect the sexuality of Ginkgo. |
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| Keywords: | Ginkgo biloba sex determination RAPD |
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