红豆草体细胞胚胎发生早期DNA,RNA和蛋白质的合成动态变化

红豆草(Onebrychis viciaefolia Scop.)下胚轴切段在含有1mg/IBA、1mg/1 KT 的 LS培养基上培养,两周后产生愈伤组织,通过筛选、克隆得到大量的具有胚胎发生潜力的非胚性愈伤组织,当将其转移到含1mg/1BA 的 LS 培养基上后可诱导体细胞胚胎发生。应用放射性同位素液体闪烁技术测得在胚性培养的前2天,RNA 合成速度迅速上升,随后下降,第五天后又呈缓慢上升趋势,尔后平稳。蛋白质合成速度在胚性培养的第三天达到高峰,升高很快。而 DNA 合成速度变化平缓,只是在胚性培养的第五天出现一较小的峰。胚性培养过程的 DNA、RNA 蛋白质合成速度均高于非胚性培养。

CHANGES IN THE SYNTHESES OF DNA,RNA AND PROTEIN DURING EARLY SOMATIC EMBRYOGENESIS IN SAINFOIN (ONOBRYCHIS VICIAEFOLIA SCOP.)

Hypocotylar explants of Onobrychis viciaefolia Scop.were cultured on LS basal medium supplemented with 1 mg/l BA and 1 mg/l KT.After two weeks of culture,calli were initia- ted on the surface of sections.Light-yellow callus from one of the explants was selected and proliferated on the medium above,Then it was transfered to LS medium with 1 mg/l BA to initiate somatic embryogenesis.The activity of RNA synthesis increased rapidly during the first two days of embryogenic culture and then decreased,but on the 5th day increased gra- dually.The activity of protein synthesis increased during the first three days and was the hi- ghest on the 3rd day.The activity of DNA synthesis had no mark change and emerged a small peak on the 5th day.All the activities of syntheses of DNA,RNA and protein were higher on embryogenic culture than on nonembryogenic culture.