| 油菜的遗传转化及抗溴苯腈转基因油菜的获得 |
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| 引用本文： | 钟蓉,朱峰.油菜的遗传转化及抗溴苯腈转基因油菜的获得[J].Acta Botanica Sinica,1997,39(1):22-27. |
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| 作者姓名： | 钟蓉 朱峰 |
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| 作者单位： | 四川联合大学植物研究所，中国科学院微生物研究所，中国科学院微生物研究所，中国科学院微生物研究所，中国科学院微生物研究所，四川联合大学植物研究所 成都 610064中国科学院成都生物研究所，成都 610041，北京 100080，北京 100080，北京 100080，北京 100080，成都 610064 |
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| 摘 要： | 以油菜(Brassica napus L.)的下胚轴和子叶为转化受体,建立了油菜的高效转化系统。在此基础上,将抗除草剂溴苯腈基因(bxn基因)导入油菜,获得了抗溴苯腈转基因油菜。分子检测实验证明,转基因油菜中含有bxn基因。转基因油菜可抗高达10~(-3)mol/L的溴苯腈。
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| 关 键 词： | 油菜 遗传转化 bxn基因 抗溴苯腈转基因油菜 |
OILSEED RAPE TRANSFORMATION AND THE ESTABLISHMENT OF A BROMOXYNIL-RESISTANT TRANSGENIC OILSEED RAPE |
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| Authors: | Zhong Rong Zhu Feng Liu Yu-le Li Sheng-guo Kang Liang-yi and Luo Peng |
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| Institution: | Zhong Rong,Zhu Feng,Liu Yu-le,Li Sheng-guo,Kang Liang-yi and Luo Peng1 |
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| Abstract: | Using hypocotyls and cotyledons as transformed materials, an efficient transformation system of oilseed rape (Brassica napus L. ) was established. Bxn gene (bromoxynil-resistant gene) was introduced into these plants, and bromoxynil-resistant transgenic oilseed rape was obtained. The molecular monitoring experiments showed that these transgenic plants contained bxn gene. The herbicide experiments showed that these transgenic plants had resistance to 10 - mol/L bromoxynil. |
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| Keywords: | Brassica napus Transformation bxn gene Bromoxynil-resistant transgenic oilseed rape |
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