| 小麦叶片老化期间的内肽酶与H_2O_2的关系(英文) |
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| 引用本文： | 张志刚,芮琪,徐朗莱.小麦叶片老化期间的内肽酶与H_2O_2的关系(英文)[J].Acta Botanica Sinica,2001(2). |
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| 作者姓名： | 张志刚 芮琪 徐朗莱 |
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| 作者单位： | 南京农业大学理学院!南京210095 |
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| 基金项目： | theNationalNaturalScienceFoundationofChina (396 70 438) |
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| 摘 要： | 研究了H2 O2 和蛋白水解酶在小麦 (TriticumaestivumL .cv.Yanmai15 8)叶片老化过程中的关系。小麦叶片老化期间 ,H2 O2 含量高的叶片中内肽酶活力也高。老化后期 ,内源H2 O2 迅速累积 ,内肽酶活力迅速上升 ;通过内肽酶同工酶电泳可检测到新增一种活力较强的内肽酶。用外源H2 O2 处理全展旗叶的内肽酶粗提液 ,随着H2 O2 浓度的升高 ,内肽酶活力先上升后下降。
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| 关 键 词： | 内肽酶 H2O2 小麦叶片 老化 |
Relationship Between Endopeptidase and H_2O_2 During the Aging of Wheat Leaf |
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| Authors: | ZHANG Zhi_Gang RUI Qi XU Lang_Lai [WTBZ] |
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| Institution: | ZHANG Zhi_Gang,RUI Qi,XU Lang_Lai [WT6BZ] |
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| Abstract: | The relationship between hydrogen peroxide (H 2O 2) and endopeptidase(EP) in wheat ( Triticum aestivum L. cv. Yanmai 158) leaves was studied during natural and artificial aging. Rapid accumulation of endogenous H 2O 2 and marked increase of EP activity were observed during the later phase of aging. A new EP isozyme with higher activity was detected by electrophoresis on polyacrylamide gels containing denatured heamoglobin. With the increase of exogenous H 2O 2, the activity of EP increased at first and then decreased. |
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| Keywords: | endopeptidase H 2O 2 wheat leaf aging |
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