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用薄层色谱分离提纯单半乳糖和双半乳糖双甘油酯
引用本文:鲍风,许永瑞,赵勤修,刘祖镗.用薄层色谱分离提纯单半乳糖和双半乳糖双甘油酯[J].Acta Botanica Sinica,1987(4).
作者姓名:鲍风  许永瑞  赵勤修  刘祖镗
作者单位:中国科学院生物物理研究所,中国科学院生物物理研究所,华南热带作物产品加工设计研究所,华南热带作物产品加工设计研究所 北京,北京
摘    要:本文选择两种溶剂体系,用两次单向薄层层析,从小麦抗寒与不抗寒品系天然橡胶胶乳分离提纯出6种单半乳糖和双半乳糖双甘油酯。并比较了它们的疏水侧链的脂肪酸组成。小麦糖脂疏水侧链脂肪酸的不饱和指数远大于天然胶乳糖脂。抗寒品系胶乳糖脂疏水侧链脂肪酸不饱和指数大于不抗寒品系。双半乳糖双甘油酯疏水侧链脂肪酸不饱和指数均大于其单半乳糖糖脂。

关 键 词:糖脂  单半乳糖双甘油酯  双半乳糖双甘油酯  小麦  天然橡胶胶乳  薄层色谱  脂肪酸

SEPARATION AND PURIFICATION OF MONOGALACTOSYL AND DIGALACTOSYL DIGLYCERIDES BY THIN-LAYER CHROMATOGRAPHY
Authors:Bao Feng Xu Yong-rui
Abstract:Six kinds of glycolipid of monogalactosyl and digalactosyl diglyceride were separated and purified from the wheat and the natural latex of chilling-tolerant and chilling-sensitive variety by two kind solvent systems and twice one-dimensional thin-layer chromatography.Besides,the compositions of fatty acid of hydrophobic side chain of six kinds of glycolipid were compared. The indices of unsaturation of fatty acid of hydrophobic side chains in the glycolipids of wheat flour,in the glycolipids of natural latex of chilling-tolerant variety and in the digalactosyl diglycerides,are much bigger than those,respectively,in the glycolipids of natural latexes,in the glycolipids of chilling-sensitive variety and in the monogalactosyl diglycerides.
Keywords:Glycolipid  Monogalactosyl diglyceride  Digalactosyl diglyceride  Wheat  Natural latex  Fatty acid  Thin-layer chromatography
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