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牛膝多糖的组分分析及抗衰老活性研究
引用本文:谈锋,邓君.牛膝多糖的组分分析及抗衰老活性研究[J].Acta Botanica Sinica,2002,44(7):795-798.
作者姓名:谈锋  邓君
作者单位:西南师范大学生命科学学院 重庆400715 (谈锋),西南师范大学生命科学学院 重庆400715(邓君)
摘    要:牛膝多糖 (Achyranthesbidentatapolysaccharides ,ABPS)是苋科植物牛膝 (AchyranthesbidentataBl.)根中的水溶性多糖 ,先后用DEAE_Sepharosefast_flow柱和SephadexG_10 0柱将牛膝粗多糖分为 4个组分 ,分别命名为 :Con .1、Con .2、Con .3和Con .4。Micro_Kjeldahl试验表明 ,Con .1中含有氮元素 3 .95 % ,另 3个小分子组分不含氮元素。以果蝇为动物模型研究了该 4个牛膝多糖组分的抗衰老作用 ,结果表明 ,大分子组分Con .1对果蝇无抗衰老作用 ,而另3个小分子量组分在培养基中浓度为 2和 5mg/g(多糖质量 /培养基质量 )时 ,都可显著或极显著地使果蝇平均体重增加 3 .85 % - 5 .47% ,并使果蝇平均寿命延长 2 .6 1% - 3.16 %。

关 键 词:牛膝  多糖  DEAE_Sepharose快速柱层析  SephadexG_100柱层析  果蝇  抗衰老作用

Analysis of the Constituents and Antisenile Function of Achyranthes bidentata Polysaccharides
Authors:TAN Feng  DENG Jun
Abstract:Achyranthes bidentata polysaccharides (ABPS), water_soluble polysaccharides, isolated from the roots of Achyranthes bidentata Bl. of Amaranthaceae family, was divided into four parts, named as Con.1, Con.2, Con.3 and Con.4, respectively, by chromatography on DEAE_Sepharose fast_flow column and Sephadex G_100 column in order. Con.1 was the constituent of high molecular weight and the other three were all of low molecular weight. Micro_Kjeldahl analysis showed that Con.1 contained 3.95% of nitrogen and neither did the other three parts. The antisenile effects of the four parts of ABPS were studied with Drosophila melanogaster. Results showed that Con.1 has no antisenile effect and all the others could significantly increase the average body weight by 3.85%-5.47% and significantly prolonged the average lifespan by 2.61%- 3.16% of D. melanogaster at the concentration of 2 or 5 mg/g (ABPS/medium).
Keywords:Achyranthes bidentata  polysaccharides  DEAE_Sepharose fast_flow column chromatography  Sephadex G_100 column chromatography  Drosophila melanogaster  antisenile effect
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