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海带,裙带菜和紫菜蛋白酶谱的研究
引用本文:王莹,李效宇.海带,裙带菜和紫菜蛋白酶谱的研究[J].Acta Botanica Sinica,1998,40(10):939-946.
作者姓名:王莹  李效宇
作者单位:青岛海洋大学海洋生命学院,河南师范大学生物系
摘    要:采用复性电泳方法对海带(LaminariaLamx.)、裙带菜(UndariaSuringar)和紫菜(PorphyraC.Ag.)3个属11种海藻的蛋白酶进行了研究,分析了海带、裙带菜和紫菜在不同pH条件下,蛋白酶种类的分布情况和活性差异。结果表明:(1)蛋白酶种类和活性受pH的影响和制约,其最适pH为8.0,此条件下蛋白酶的种类最多,活性最强;(2)亲缘关系越近,蛋白酶种类和活性越相似,海带同属不同种之间存在相同的蛋白酶,而不同属的海藻中很难找到相同的蛋白酶带;(3)海带属不同种所共有的两种蛋白酶(分子量19kD和18kD)是其特征性酶带;(4)蛋白酶谱可作为海藻分类依据之一。

关 键 词:海带,裙带菜,紫菜,蛋白酶,pH,复性电泳,分类学

SPECTRAL PROFILES ON THE PROTEASE ACTIVITIES IN LAMINARIA, UNDARIA AND PORPHYRA
Authors:WANG Ying  LI Xiao Yu  XU Cun Shuan  DAI Ji Xun
Institution:1WANG Ying 2LI Xiao Yu 2XU Cun Shuan 1DAI Ji Xun 1
Abstract:G PAGE was used to investigate the protease patterns and activities in 11 species of Laminaria Lamx., Undaria Suringar and Porphyra C.Ag. It was shown that (1) the patterns and activities of the protease were pH dependent and the optimum pH for protease activities was 8.0; (2) the more closer the inter species relationship, the more similarities were observed among the patterns and activities of the proteases; (3) two protease patterns (19 kD,18 kD) in the eight species of Laminaria were regarded as the genetic indicator of Laminaria; (4) the results suggested that the G PAGE analysis of proteases could be valuable in the systematic taxonomic study of algae.
Keywords:Laminaria  Undaria  Porphyra  Protease  pH  G  PAGE  Taxonomy  
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