| 一种简单快速测定Rubisco CO2／O2特异性因子的方法 |
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| 引用本文： | 李国富,陆巍.一种简单快速测定Rubisco CO2／O2特异性因子的方法[J].Acta Botanica Sinica,2000,42(12):1304-1307. |
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| 作者姓名： | 李国富 陆巍 |
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| 作者单位： | [1]清华大学生物科学与技术系生物膜与膜生物工程国家重点实验 [2]南京农业大学水稻研究所 |
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| 基金项目： | TheStateKeyBasicResearchandDevelopmentPlan (G19980 10 10 0 )，TheNationalNaturalScienceFoundationofChina . |
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| 摘 要： | 提出了一种简单快速测定１，５－二磷酸核酮糖羧化／氧化酶ＣＯ２／Ｏ２特异性因子的方法。理论上改进了定量计算公式；操作上避免了使用放射性同位素标记以及层析分离３－磷酸甘油酸和２－磷酸乙醇酸的复杂程度，使测定过程一步完成，极大地减少了随机误差。讨论了实验数据（ｐＨ、温度、离子强度）的准确性对计算结果的影响。
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| 关 键 词： | CO2／O2特异性因子 准确性 酶 快速测定 Rubisco |
A Simple and Rapid Method to Determine the CO_2/O_2 Specificity Factor for Rubisco |
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| Authors: | LI Guo_Fu LU Wei LI Sen GONG Yan_Dao ZHANG Xiu_Fang ZHANG Rong_Xian ZHOU Hai_Meng ZHAO Nan_Ming [WTBZ] |
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| Institution: | LI Guo_Fu 1,LU Wei 2,LI Sen 1,GONG Yan_Dao 1,ZHANG Xiu_Fang 1,ZHANG Rong_Xian 2,ZHOU Hai_Meng 1,ZHAO Nan_Ming 1 * [WT6BZ] |
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| Abstract: | A simple and rapid new method is presented to determine the CO 2/O 2 specificity factor for ribulose_1,5_bisphosphate(RuBP) carboxylase/oxygenase (Rubisco). In principle, the quantitative formula, which intrinsically resulted in error or enormous error in all reported methods, was improved to circumvent the problem. In practice, the complicated procedure with radiolabelling and chromatographic separation of 3_phosphoglycerate and 2_phosphoglycolate was substituted by one_step procedure without radiolabelling and chromatographic separation to decrease the random error. The effects of pH, temperature and ionic strength on accuracy of the data and the reliability of the calculation of the CO 2/O 2 specificity factor are also discussed. |
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| Keywords: | Rubisco CO 2/O 2 specificity factor error accuracL |
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