高山红景天细胞悬浮培养中pH值对红景天甙胞外释放及细胞活性的影响

高山红景天(Rhodiola sachalinensis A.Bor.)细胞悬浮培养中,通过降低培养基pH值能有效地诱导培养细胞中红景天甙的胞外释放。红景天甙的跨膜运输是一个与H~ 对运的动态过程,培养基pH值决定了红景天甙在胞内外含量的分布。细胞组织在pH值大于3的培养基中处理3h以内,对细胞活性的影响不大。将诱导释放处理过的细胞组织转入到新鲜的生产培养基中,细胞仍具有合成红景天甙的能力。

EFFECTS OF MEDIUM pH DECREASE ON SALIDROSIDE RELEASE AND CELL VIABILITY IN CELL SUSPENSION CULTURE OF RHODIOLA SACHALINENSIS

The release of intracellular salidroside could be effectively induced by decreasing the medium pH in cell suspension culture of Rhodiola sachalinensis A. Bor. The transport of salidroside across the membrane was a dynamic process with an H -antiport mechanism, in which the ratio of intra- and extra-cellular salidroside content was medium pH dependant. If the cultured cells were induced to release salidroside in a medium with a constant pH of 3 for less than 3 h, the growth ability of the cells were retained. When transferred to the productive medium, the treated cells still sustained their ability of synthesizing salidroside.