| 抗阿特拉津转基因大豆植株后代的遗传分析 |
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| 引用本文： | 岳绍先,刘博林.抗阿特拉津转基因大豆植株后代的遗传分析[J].Acta Botanica Sinica,1990,32(5):343-349. |
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| 作者姓名： | 岳绍先 刘博林 |
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| 作者单位： | 中国农业科学院作物育种栽培研究所，中国科学院植物研究所，中国科学院植物研究所，中国科学院遗传研究所，中国科学院遗传研究所，中国农业科学院作物育种栽培研究所，中国农业科学院作物育种栽培研究所，中国科学院遗传研究所 北京 100081。，北京 100044，北京 100044，北京 100012，北京 100012，北京 100081，北京 100081，北京 100012 |
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| 摘 要： | 本试验用阿特拉津溶液涂抹、荧光诱导动力学检测、分子杂交等方法对抗阿特拉津转基因大豆植株的后代进行了鉴定,在第二代及第三代中检测到了抗性基因的存在,表明从龙葵中得到的此抗阿特拉津 psbA 基因不仅能导人大豆叶绿体基因组中获得表达,而且可以遗传到后代。
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| 关 键 词： | 大豆 阿特拉津 转基因植株 |
A GENETIC ANALYSIS TO THE PROGENIES OF ATRAZINE-RESISTANCE TRANSGENIC SOYBEAN PLANT |
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| Authors: | Yue Shao-xian Liu Bo-lin Mao Da-zhang Li Xiao-bing Hu Nai-bi Fu Jun-hua Li Lian-cheng Zhu Li-huang Institute of Crop Breeding & Cultivation Chinese Academy of Agricultural Sciences Beijing |
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| Institution: | Yue Shao-xian Liu Bo-lin Mao Da-zhang Li Xiao-bing Hu Nai-bi Fu Jun-hua Li Lian-cheng Zhu Li-huang *Institute of Crop Breeding & Cultivation,Chinese Academy of Agricultural Sciences,Beijing 100081.**Institute of Botany,Acadtmia Sinica,Beijing 100044.***Institute Of Genetics,Academia Sinica,Beijing 100012. |
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| Abstract: | The identification of the progenies of atrazine-resistante transgenic soybeen plants was carried out by using the methods of spraying the leaves directly with atrazine solution,ex- amining the change of leaf fluorescence kinetics curve under a brighter light induction and molecular hybridization.The resistant gene has been found in plants of F_2 and F_3 generations, it means that the atrazine-resistance psbA gene from black nightshade not only can be trans- ferred to the chloroplast genome of atrazine-susceptible soybean and expressed,but also inherit- ed to the progenies. |
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| Keywords: | Atrazine psbA gene Transgenic plant Soybean Black nightshade |
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