The effects of ethyl methanesulfonate treatment on <Emphasis Type="Italic">Eucalyptus</Emphasis> pollen behaviour in vitro |
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| Authors: | L J McManus J Sasse C K Blomstedt G Bossinger |
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| Institution: | (1) School of Forest and Ecosystem Science, University of Melbourne, Water Street, Creswick, Victoria, 3363, Australia;(2) Sassafras Group, 2 O’Farrell Street, Yarraville, Victoria, 3013, Australia;(3) Present address: School of Biological Sciences, Monash University, Clayton, Victoria, 3800, Australia |
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| Abstract: | Treating pollen with mutagens prior to controlled pollination may facilitate the production of mutant trees for developmental
studies and eventual plantation improvement. To establish a suitable dose of the chemical mutagen ethyl methanesulfonate (EMS)
for the testing of this hypothesis, pollen of Eucalyptus globulus ssp. globulus and E. grandis was studied in vitro. Pollen germination, pollen tube elongation and generative cell division were examined after 48 h of
culture, following exposure to between 0 and 1,000 ppm EMS. Doses of 600 to 1,000 ppm EMS reduced pollen germination in vitro
in both species. Doses of up to 1,000 ppm EMS were not observed to significantly impact on either pollen tube length, or generative
cell division in vitro of either species. A dose of 600 ppm EMS in paraffin oil is predicted to induce mutation in Eucalyptus species whilst impacting minimally on seed production based on the effect on pollen germination. |
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| Keywords: | EMS Tree mutagenesis Pollen culture Generative nuclei Generative cell division |
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