Preparation of Oligonucleotides Containing 5-Bromouracil and 5-Methylcytidine. |
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| Authors: | E Ferrer C Fàbrega R G Garcia F Azorín R Eritja |
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| Institution: | 1. Centro de Investigación y Desarrollo , CSIC. Jordi Girona 18, Barcelona, 08034, Spain;2. European Molecular Biology Laboratory (EMBL). , Meyerhofstrasse 1, D-69117, Heidelberg, Germany;3. European Molecular Biology Laboratory (EMBL). , Meyerhofstrasse 1, D-69117, Heidelberg, Germany;4. Centro de Investigación y Desarrollo , CSIC. Jordi Girona 18, Barcelona, 08034, Spain |
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| Abstract: | Abstract A previously described side reaction on 5-bromouracil during standard oligonucleotide deprotection conditions has been studied in detail. The side product, 5-amino-2′-deoxyuridine, is isolated and characterized. The use of several 5-methylcytidine protected derivatives for the preparation of oligonucleotides containing 5-bromouracil and 5-methylcytidine free of 5-amino-2′-deoxyuridine is discussed. |
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