Oligonucleotide Directed Mutagenesis of the E. coli hisS Gene: Introduction of an Ncoi Restriction Site at the Initiating GTG and Cloning of the Mutated Gene to the Expression Vector pKK 233–2 |
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| Authors: | Thomas Glade Uwe Englisch |
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| Institution: | Max-Planck-Institut für experimentelle Medizin, Abteilung Chemie , Hermann-Rein-Str. 3, D-3400, Gottingen, F.R.G. |
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| Abstract: | Abstract Site directed mutagenesis of the E. coli his gene with a double mismatch primer changed the initiation codon GTG to ATG and introduced an Ncol restriction site at the start codon. The promoter-deleted structural gene was cloned to the expression vector pKK 233–2. |
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