Determination of 5-Fluorouracil in Plasma with HPLC-Tandem Mass Spectrometry |
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| Authors: | A B P van Kuilenburg H van Lenthe J G Maring A H van Gennip |
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| Institution: | 1. Academic Medical Center , University of Amsterdam, Emma Children's Hospital and Department of Clinical Chemistry , Amsterdam, the Netherlands;2. Diaconessen Hospital Meppel, Department of Pharmacy , Meppel, the Netherlands;3. Academic Hospital Maastricht, Departments of Clinical Genetics and Clinical Chemistry , Maastricht, the Netherlands |
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| Abstract: | In this article, we describe a fast and specific method to measure 5FU with HPLC tandem-mass spectrometry. Reversed-phase HPLC was combined with electrospray ionization tandem mass spectrometry and detection was performed by multiple-reaction monitoring. Stable-isotope-labeled 5FU (1,3–15N2–5FU) was used as an internal standard. 5FU was measured within a single analytical run of 16 min with a lower limit of detection of 0.05 μM. The intra-assay variation and inter-assay variation of plasma with added 5FU (1 μM, 10 μM, 100 μM) was less then 6%. Recoveries of the added 5FU in plasma were > 97%. Analysis of the 5FU levels in plasma samples from patients with the HPLC tandem mass spectrometry method and a HPLC-UV method yielded comparable results (r2 = 0.98). Thus, HPLC with electrospray ionization tandem mass spectrometry allows the rapid analysis of 5FU levels in plasma and could, therefore, be used for therapeutic drug monitoring. |
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| Keywords: | 5-Fluorouracil Dihydropyrimidine dehydrogenase Pharmacokinetics |
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