Effect of AMP-Deaminase 3 Knock-Out in Mice on Enzyme Activity in Heart and Other Organs |
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| Authors: | Iwona Rybakowska Pawel Romaszko Magdalena Zabielska Jacek Turyn Krystian Kaletha Paul J Barton |
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| Institution: | 1. Department of Biochemistry and Clinical Physiology, Medical University of Gdansk, Gdansk, Polandiwonar@gumed.edu.pl;3. Department of Biochemistry, Medical University of Gdansk, Gdansk, Poland;4. Department of Biochemistry and Clinical Physiology, Medical University of Gdansk, Gdansk, Poland;5. National Heart and Lung Institute, Imperial College, London, United Kingdom |
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| Abstract: | Recent findings suggest that inhibition of AMP-deaminase (AMPD) could be effective therapeutic strategy in heart disease associated with cardiac ischemia. To establish experimental model to study protective mechanisms of AMPD inhibition we developed conditional, cardiac specific knock-outs in Cre recombinase system. AMPD3 floxed mice were crossed with Mer-Cre-Mer mice. Tamoxifen was injected to induce Cre recombinase. After two weeks, hearts, skeletal muscle, liver, kidney, and blood were collected and activities of AMPD and related enzymes were analyzed using HPLC-based procedure. We demonstrate loss of more than 90% of cardiac AMPD activity in the heart of AMPD3 -/- mice while other enzymes of nucleotide metabolism such as adenosine deaminase, purine nucleoside phosphorylase were not affected. Surprisingly, activity of AMPD was also reduced in the erythrocytes and in the kidney by 20%–30%. No change of AMPD activity was observed in the skeletal muscle and the liver. |
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| Keywords: | AMP-deaminase Cre recombinase AMPD3 wild type AMPD3 homozygous cardioprotection |
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