Characterization of Interrelated Sequence Motifs in Four Satellite DNAs and Their Distribution in the Genome of the Mollusc Donax trunculus |
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Authors: | Miroslav Plohl Luis Cornudella |
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Institution: | (1) Departamento de Biología Molecular y Celular, Centro de Investigación y Desarrollo del C.S.I.C., and Unitat de Biologia Molecular del Centre de Referència en Biotecnologia de la Generalitat de Catalunya, Jordi Girona, 18-26, E-08034 Barcelona, Spain, ES |
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Abstract: | A family of four satellite DNAs has been characterized in the genome of the bivalve mollusc, Donax trunculus. All share HindIII sites, a similar monomer length of about 160 base pairs (bp), and the related oligonucleotide motifs GGTCA and GGGTTA,
repeated six to 15 times within the repetitive units. The motif GGTCA is common to all members of the satellite family. It
is present in three of them in both orientations, interspersed within nonrepetitive DNA sequences. The hexanucleotide GGGTTA
appears to be the main building element of one of the satellites forming a prominent subrepeat structure in conjunction with
the 5-bp motif. The former has been also found in perfect tandem repeats in a junction region adjacent to the proper satellite
sequence. Southern analysis has revealed that (GGGTTA)n and/or related sequences are abundant and widely distributed in the D. trunculus genome. The distribution observed is consistent with the concurrence of the scattering of short sequence motifs throughout
the genome and the spread of longer DNA segments, with concomitant formation of satellite monomer repeats. Both kinds of dispersion
may have contributed to the observed complex arrangement of the HindIII satellite DNA family in Donax.
Received: 28 May 1996 / Accepted: 30 July 1996 |
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Keywords: | :HindIII restriction — Repetitive subfamilies — Sequence motifs — Genomic distribution — Molluscs |
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