Higher-Order Organization of Subrepeats and the Evolution of Cervid Satellite I DNA |
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Authors: | Charles Lee Dean R Court Charles Cho Jennifer L Haslett Chyi-Chyang Lin |
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Institution: | (1) Department of Laboratory Medicine and Pathology, University of Alberta, Edmonton, Alberta, Canada, T6G 2B7, CA |
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Abstract: | Based on sequence analyses of 17 complete centromeric DNA monomers from ten different deer species, a model is proposed for
the genesis, evolution, and genomic organization of cervid satellite I DNA. All cervid satellite I DNA arose from the initial
amplification of a 31-bp DNA sequence. These 31-bp subrepeats were organized in a hierarchical fashion as 0.8-kb monomers
in plesiometacarpalia deer and 1-kb monomers in telemetacarpalia deer. The higher-order repeat nature of cervid centromeric
satellite DNA monomers accounts for their high intragenomic and intraspecific sequence conservation. Such high intraspecific
sequence conservation validates the use of a single cervid satellite I DNA monomer from each deer species for interspecific
sequence comparisons to elucidate phylogenetic relationships. Also, a specific 0.18-kb tandem duplication was observed in
all 1-kb monomers, implying that 1-kb cervid satellite I DNA monomers arose from an unequal crossover event between two similar
0.8-kb ancestral DNA sequences.
Received: 28 May 1996 / Accepted: 24 October 1996 |
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Keywords: | : Cervid satellite DNA — Centromere — Subrepeats — Monomers — Higher-order repeats |
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