Effects of endothelin family on ANP secretion |
| |
| Institution: | 1. Department of Physiology, Chonbuk National University Medical School, Jeonju 560-180, Republic of Korea;2. Department of Surgery, Chonbuk National University Medical School, Jeonju 560-180, Republic of Korea;3. Department of Internal Medicine, Yanbian University, China;1. Instituto de Microscopía Electrónica y Materiales, Departamento de Ciencia de los Materiales e I.M. y Q.I., Facultad de Ciencias, Universidad de Cádiz, Campus Río San Pedro, s/n, Puerto Real, Cádiz 11510, Spain;2. School of Engineering, University of Glasgow, Glasgow G12 8QQ, UK;3. GPM, Université et INSA de ROUEN, UMR CNRS 6634, BP 12, Avenue de l’université, Saint Etienne du Rouvray 76801, France;1. LIMOS UMR CNRS 6158, Université Blaise Pascal, F-63 173 Aubière, France;2. Institut für Informatik, Universität Rostock, D-18051 Rostock, Germany;3. MIS, Université de Picardie Jules Verne, Amiens, France;4. CNRS, G-SCOP, Grenoble-INP, Université Joseph Fourier, Grenoble, France;1. Servicio de Urgencias, Hospital Universitari de Bellvitge, IDIBELL, L’Hospitalet de Llobregat, Barcelona, España;2. Servicio de Urgencias, Unidad de Corta Estancia y Unidad de Hospitalización a Domicilio, Hospital Universitario General de Alicante, Alicante, España;3. Servicio de Urgencias, Hospital Clinic de Barcelona, Grupo de Investigación «Urgencias: procesos y patologías», IDIBAPS, Barcelona, España;1. Department of Neurosurgery, Cannizzaro Hospital, Trauma Center, Gamma Knife Center, Catania, Italy;2. Department of Neurosurgery, Highly Specialized Hospital of National Importance “Garibaldi”, Catania, Italy;3. Division of Neurosurgery, Department of Biomedical and Dental Sciences and Morpho-Functional Imaging, University of Messina, Messina, Italy |
| |
| Abstract: | The endothelins (ET) peptide family consists of ET-1, ET-2, ET-3, and sarafotoxin (s6C, a snake venom) and their actions appears to be different among isoforms. The aim of this study was to compare the secretagogue effect of ET-1 on atrial natriuretic peptide (ANP) secretion with ET-3 and evaluate its physiological meaning. Isolated nonbeating atria from male Sprague-Dawley rats were used to evaluate stretch-activated ANP secretion in response to ET-1, ET-2, ET-3, and s6C. Changes in mean blood pressure (MAP) were measured during acute injection of ET-1 and ET-3 with and without natriuretic peptide receptor-A antagonist (A71915) in anesthetized rats. Changes in atrial volume induced by increased atrial pressure from o to 1, 2, 4, or 6 cm H2O caused proportional increases in mechanically-stimulated extracellular fluid (ECF) translocation and stretch-activated ANP secretion. ET-1 (10 nM) augmented basal and stretch-activated ANP secretion in terms of ECF translocation, which was blocked by the pretreatment with ETA receptor antagonist (BQ123, 1 μM) but not by ETB receptor antagonist (BQ788, 1 μM). ETA receptor antagonist itself suppressed stretch-activated ANP secretion. As compared to ET-1- induced ANP secretion (3.2-fold by 10 nM), the secretagogue effects of ANP secretion by ET-2 was similar (2.8-fold by 10 nM) and ET-3 and s6C were less potent (1.7-fold and 1.5-fold by 100 nM, respectively). Acute injection of ET-1 or ET-3 increased mean blood pressure (MAP), which was augmented in the presence of natriuretic peptide receptor-A antagonist. Therefore, we suggest that the order of secretagogue effect of ET family on ANP secretion was ET-1 ≥ ET-2 >> ET-3 > s6C and ET-1-induced ANP secretion negatively regulates the pressor effect of ET-1. |
| |
| Keywords: | Atrial natriuretic peptide Endothelin Stretch Atrium Receptor Blood pressure |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
