盘基网柄菌DdLIS1蛋白生物信息学分析

LIS1蛋白是一种与人类无脑回疾病以及细胞癌变相关的重要蛋白。对盘基网柄菌DdLIS1进行生物信息学分析,探究盘基网柄菌能否作为研究人类无脑回疾病及细胞癌变机制的模型。现从NCBI中的Genank找到盘基网柄菌DdLIS1的氨基酸序列,随后进行blastp找到模式生物中相似序列,利用理化性分析网站ProtScale、ProtParam分析DdLIS1的理化性质,通过NCBI中的保守结构域库(CDD)分析DdLIS1的保守结构域,使用MEGA6.0并选用邻位连接法构建系统进化树,分别使用PredictProtein、SWISS-MODEL网站预测Dd LIS1蛋白的二级结构、三维结构。结果得出DdLIS1蛋白全长为419,属于亲水性蛋白,有7个保守结构域,属于WD40家族,与人类和小鼠的氨基酸序列相似性为72%。二级结构中β折叠所占比例最高,为49.40%,α螺旋、随机卷曲分别占该蛋白7.16%、43.44%,与三级结构一致。以上结果说明DdLIS1与LIS1高度相似,有助于盘基网柄菌能够作为研究人类无脑回疾病以及细胞癌变机制的模型。

Bioinformatics Analysis of DdLIS1 Protein in Dictyostelium discoideum

LIS1 protein is an important protein related to human lissencephaly disease and cell canceration. This study carried out the bioinformatics analysis of DdLIS1, and explored whether Dictyostelium discoideum could be used as a model for the study of human lissencephaly disease and cell canceration. The amino acid sequence of Dictyostelium discoideum DdLIS1 was obtained from Genank in NCBI. Then, the similar sequences of model organisms were found by blastp. Physicochemical properties of DdLIS1 were analyzed by the websites of ProtScale and ProtParam. The conserved domain database(CDD) in NCBI was used to analyze the conserved domain of DdLIS1. The phylogenetic tree was constructed by the orthotopic linkage method of MEGA6.0. PredictProtein and SWISS-MODEL were respectively used to predict the secondary and the tertiary structure of DdLIS1. The results showed that the full length of DdLIS1 protein was 419, which belonged to the hydrophilic protein and had seven conserved domains belonging to the WD40 family. The similarity of DdLIS1 with LIS1 of Homo sapiens and Mus musculus was 72%. In DdLIS1, the proportion of β-fold in the secondary structure was the highest, 49.40%,α-helix and the random curl respectively accounted for 7.16% and 43.44%, which was consistent with the tertiary structure. These results indicate that DdLIS1 might be highly similar to LIS1, and it could be beneficial to study the human lissencephaly disease and cell canceration.