嗜麦芽寡养单胞菌全基因组测序及降解荧蒽特性研究

随着当前工业化社会的发展,由多环芳烃等石油污染物造成的土壤污染已经成为了世界性的环境问题。本实验在天津滨海石油污染场地分离筛选出一株多环芳烃高效降解菌株W18,经过细胞形态、理化实验和分子生物学鉴定该菌株为嗜麦芽寡养单胞菌(Stenotrophomonas maltophilia)。在表面活性剂吐温80诱导下,该菌对荧蒽降解率可达到73%。利用细菌全基因组De novo测序技术发现,其包括1个环状拓扑结构,基因大小为4 738 432 bp,GC含量为66.69%。通过与主要数据库(COG,GO,KEGG)进行比对注释,发现W18菌株中有25个基因编码了与PAHs降解有关的加氧酶。嗜麦芽寡养单胞菌为土壤石油污染处理的研究提供菌种资源,对其降解功能基因的研究将为微生物-多环芳烃高效修复体系提供重要的理论依据。

Whole-genome Sequencing of S. maltophilia and Degradation of Fluoranthene

With the development of industrial society,soil pollution caused by polycyclic aromatic hydrocarbons(PAHs)has become a worldwide environmental problem.In this study,PAHs efficient degradation strain W18 was isolated from the soil of the oil-polluted site of Tianjin Binhai.After cell morphology,physical and chemical experiments and molecular biology,the strain was identified as Stenotrophomonas maltophilia.The degradation rate of fluoranthene by Tween 80 was 73%.The W18 was sequenced by bacterial whole genome De novo sequencing technology and found to include a circular topology with a gene size of 4 738 432 bp and a GC content of 66.69%.By comparing the annotations with the main databases(COG,GO,KEGG),it was found that 25 of the W18 strains encode oxygenases involved in the degradation of PAHs.Stenotrophomonas maltophilia will provide strain resources for the study of soil oil pollution treatment,and the study of its degradation functional genes will provide an important theoretical basis for the efficient Microbial-PAHs restoration system.