都柳江南方白甲鱼群体mtDNA D-loop序列及遗传多样性分析

为探究都柳江流域南方白甲鱼种群种质资源现状。本研究以采集自都柳江的40尾南方白甲鱼个体为实验样本,使用mtDNA D-loop分子标记技术,得到了长度近700 bp的南方白甲鱼D-loop序列。经分析序列中碱基A、T的含量高于碱基C、G,识别了序列的保守序列区、终止序列区和中央保守区以及相关的核心序列。都柳江南方白甲鱼群体D-loop序列有变异点13个,变异类型包括缺失、插入、转换和颠换。多态位点11个,其中单突变点和简约信息点分别有3个和8个。碱基转换颠换比为4.5∶1,变异尚未达饱和。确定了15个单倍体型。都柳江南方白甲鱼群体D-loop序列单倍型多样性度和核苷酸多样性指数分别为0.8777和0.0033。都柳江南方白甲鱼群体近期可能存在扩张现象,但其核苷酸多样性较低,需要开展其种质资源研究和保护。本研究从分子水平上为都柳江南方白甲鱼种质资源的评价、开发和利用提供基础。

Analysis of D-loop Sequence and Genetic Diversity for Onychostoma gerlachi Population in Duliu River

In order to explore the present situation of germplasm Onychostoma gerlachi population in Duliu River Basin.On the basis of 40 individuals of Onychostoma gerlachi from the Duliu River,Guizhou,a total of about 700 bp D-loop sequence in mtDNA of the species was obtained,through the method of DNA sequencing.In the sequence,the contents of bases A,T were significantly higher than those of bases G,C.The termination associated sequence(ETAS),central conserved domain(CD),conserved sequence block(CSB)and related core sequences were identified in the D-loop sequence.There were 13 variable loci including deletion,insertion,transition and transversion,and 11 Polymorphic loci comprising 3 singleton variable sites and 8 parsimony informative sites were detected in the sequence.The mutation of DNA was unsaturated for the transition/transversion(Ts/Tv)of bases in the D-loop sequence was 4.5.The 40 individuals belonged to 15 haplotypes according to the determined sequences.The haplotypic diversity(Hd)of the population was 0.8777.And its nucleotide diversity(π)was 0.0033.The population of Onychostoma gerlachi from the Duliu River might experience quantity expansion in the near past but still remained much low nucleotide diversity and therefore it is urgent to carry out the protection of the population to increase its genetic diversity.This study would provide useful information for evaluation,development and utilization of Onychostoma gerlachi germplasm resources in the Duliu River.