高强度间歇运动对脑梗死后大鼠的脑保护作用及HDAC6表达的影响

为了考察高强度间歇运动(HIIE)对脑梗死后大鼠的脑保护作用及组蛋白去乙酰化酶6 (HDAC6)表达的影响。本研究将60只SD大鼠随机分为3组,假手术组、大脑中动脉闭塞模型组和HIIE组,每组20只。HIIE组大鼠在建模48 h后进行4周的高强度间歇运动,其他组大鼠不进行运动。通过神经损伤评分来评价大鼠神经功能,TTC染色检测梗死面积,TUNEL染色测定脑组织的细胞凋亡,RT-PCR和Western blotting检测大鼠海马组织中HDAC6、TNF-α、IL-1β和IL-6的m RNA和蛋白表达。研究发现,高强度间歇运动后,HIIE组的神经损伤评分显著低于模型组(p<0.05)。TTC染色显示,HIIE组的梗死面积比例显著低于模型组(p<0.05)。TUNEL染色显示,HIIE组的海马神经细胞凋亡数显著低于模型组(p<0.05)。RT-PCR和Western blotting结果显示,HIIE组的HDAC6、TNF-α、IL-1β和IL-6 m RNA和蛋白表达水平均显著低于模型组(p<0.05)。本研究表明,高强度间歇运动可显著改善脑梗死大鼠的神经功能,降低脑梗死面积,抑制海马神经细胞凋亡。高强度间歇运动的神经保护作用机制可能与抑制HDAC6有关。

Effects of High-Intensity Intermittent Exercise on Brain Protection and HDAC6 Expression in Rats after Cerebral Infarction

To investigate the effects of high-intensity intermittent exercise(HIIE) on cerebral protection and the expression of histone deacetylase 6(HDAC6) in rats after cerebral infarction,60 SD rats were randomly divided into three groups,sham group,middle cerebral artery occlusion model group and HIIE group,with 20 rats in each group.Rats in the HIIE group performed high-intensity intermittent exercise for 4 weeks after modeling for 48 hours,while the other groups did not exercise.Nerve function was evaluated by nerve injury score.TTC staining was used to detect infarct size.TUNEL staining was used to determine brain tissue apoptosis.RT-PCR and Western blotting were used to detect HDAC6,TNF-α,IL-1β and IL-6 mRNA and protein expression in rat hippocampus.The study found that after high-intensity intermittent exercise,the nerve injury score in the HIIE group was significantly lower than that in the model group(p<0.05).TTC staining showed that the proportion of infarct area in the HIIE group was significantly lower than that in the model group(p<0.05).TUNEL staining showed that the number of hippocampal neuronal apoptosis in the HIIE group was significantly lower than that in the model group(p<0.05).RT-PCR and Western blotting results showed that HDAC6,TNF-α,IL-1β and IL-6 mRNA and protein expression levels in the HIIE group were significantly lower than those in the model group(p<0.05).This study demonstrates that high-intensity intermittent exercise could significantly improve the neural function of cerebral infarction rats,reduce the area of cerebral infarction,and inhibit hippocampal neuronal apoptosis.The neuroprotective mechanism of high-intensity intermittent exercise might be related to the inhibition of HDAC6.