粗甘油优化污泥脱水液培养小球藻过程的研究

污泥脱水液为污泥压缩过程产生的污水,因其含有N、P等营养物,可用于微藻的培养。但污泥脱水液碳氮比低,可利用碳源有限,影响微藻生长。本研究考察外加不同浓度(1 g/L,2 g/L,4 g/L,6 g/L)生物柴油副产物-粗甘油对污泥脱水液培养小球藻过程的影响。结果表明:1 g/L、2 g/L粗甘油浓度能促进小球藻生长,藻生物量为1.29 g/L、1.45 g/L;2 g/L粗甘油浓度下氨氮、总氮去除率达99.32%和97.52%。粗甘油被分解后易使培养体系pH降至7以下,使总磷去除率比对照组略低。外加1 g/L、2 g/L粗甘油组的COD去除量分别为553.00 mg/L和405.00 mg/L。藻细胞元素和傅里叶红外光谱分析表明补加粗甘油后藻细胞中C元素和H元素相对含量均明显增加,C元素含量约为对照组的1.5倍;2 g/L粗甘油组的蛋白质与脂类物质含量均高于对照组。MPBR半连续培养小球藻过程中,HRT为5 d时藻生物量维持在1.99~2.21 g/L,大约为批次生物量的1.50倍;氨氮、总氮、总磷、COD的去除率分别在96.26%~99.20%、92.44%~94.04%、53.63%~58.58%、59.44%~65.57%。

Study on Optimization of Chlorella vulgaris Cultivation Process in Centrate by Crude Glycerol

Microalgae could be cultivated in the centrate,which was generated through dewatering sludge and contained nutrient(N,P).However,there was insufficient carbon source to sustain algae growth in the centrate due to the low carbon to nitrogen ratio.This study assessed the process of cultivating Chlorella vulgaris with byproduct of biodiesel crude glycerol(1 g/L,2 g/L,4 g/L,6 g/L) in centrate.The results showed 1 g/L and 2 g/L concentration of crude glycerol could accelerate algae growth and the biomass were 1.29 g/L and 1.45 g/L,respectively.The removal efficiencies of ammonia nitrogen and total nitrogen were 99.32% and 97.52% in 2 g/L concentration of crude glycerol.It was easy to make the pH of the culture system to under 7 during decomposition and utilization of crude glycerol.The removal efficiency of phosphorus in crude glycerol groups were lower than control group due to the low pH.The removal efficiencies of COD were 553.00 mg/L and 405.00 mg/L after adding 1 g/L,2 g/L crude glycerol,respectively.The analysis of algal cell elements and FTIR showed the element of C and H were much higher after adding crude glycerol,the element of C in algae cells after adding crude glycerol was 1.50 times to control group.The protein and lipid in 2 g/L crude glycerol were higher than control group.Chlorella vulgaris was semi-continuously cultured in MPBR.The range of biomass was 1.99~2.21 g/L when HRT was 5 d and that were about 1.50 times of batch group.The range of NH4+-N,TN,TP and COD removal efficiencies were 96.26%~99.20%,92.44%~94.04%,53.63%~58.58%,59.44%~65.57%,respectively.