SSR标记鉴定浙江省主要无性系茶树品种的研究

为促进浙江省茶树育种的发展,利用SSR引物对浙江省茶树育成品种的遗传多样性进行了研究,筛选出可用于鉴别浙江省茶树品种的核心鉴定引物和标准品种,并进一步应用于未知茶苗身份鉴定。首先,利用35对SSR引物研究了36个茶树育成品种,并进行聚类分析;然后,根据电泳谱带和基因型筛选出核心鉴定引物和标准品种;最后,对4株未知茶苗进行了身份鉴定。结果表明:共有34对引物表现出多态性,各品种基本按遗传背景聚类,重复样本间遗传距离介于0~0.094;有10对引物确定为核心鉴定引物,8个品种为标准品种;4株未知身份茶苗中,NH-01属于乌牛早品种,另外3株并非浙江现有品种。本研究认为,核心鉴定引物在两个浙江育成品种间差异引物对≥2时,应判定为不同品种;差异引物对≤1时,应判定为相同品种或极相似品种,必要时应引入其余24对引物计算遗传距离进一步验证,遗传距离>0.140判定为不同品种,遗传距离≤0.140判定为同一品种。

Application of SSR markers in cultivar identification of clonal tea plant in Zhejiang Province, China

Abstract: Thirty six tea cultivars origin from Zhejiang province were analyzed using SSR markers. The genetic diversity and cluster analysis shown that: totally 121 alleles and 187 genotypes were amplified by 34 polymorphism markers, with an average of 3.5 and 5.5, respectively; the polymorphism information content (PIC) were ranged from 0.02 to 0.74, average 0.45; most similar cultivars were classified according to their genomic background. Ten core SSR markers fitting for cultivar identification were confirmed, and were used to evaluate 4 unknown varieties. It is believed that if more than 2 core primer pairs were different between 2 tea plants, they should be defined as different varieties; otherwise they are same variety or highly similarity varieties. If necessary, the value of genetic distance should be used to distinguish highly similarity varieties, if the value >0.140 they should be defined as different varieties, otherwise they are same variety. We believe that, this study will promote the breeding research of tea plant in Zhejiang province.