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| 非洲紫罗兰叶片体细胞胚培养及快繁技术研究 | |
| 引用本文： | 师嘉临,师桂英,任治斌,汉梅兰,师春娟.非洲紫罗兰叶片体细胞胚培养及快繁技术研究[J].植物遗传资源学报,2005,6(4):453-456. |
| 作者姓名： | 师嘉临 师桂英 任治斌 汉梅兰 师春娟 |
| 作者单位： | 1. 全国贫困地区干部培训中心,兰州,730070 2. 甘肃农业大学园艺系,兰州,730070 3. 兰州市南北两山环境绿化工程指挥部,兰州,730000 4. 兰州市园林科学研究所,兰州,730070 5. 甘肃省小陇山林业科学研究所,天水,741026 |
| 摘 要： | 以非洲紫罗兰叶片为材料进行胚状体诱导及快繁技术研究.结果表明:.在MS NAA0.1mg/L BA0.1 mg/L 2,4-D1.0 mg/L的培养基上培养15d利于诱导胚性细胞分化,起始黑暗培养5～10d可提高胚性细胞分化率;在MS BA0.05～1mg/L的培养基上可诱导胚状体大量发生;在MS NAA0.1mg/L十BA0.1 mg/L的培养基上能够获得茎芽快速增殖;在1/2MS NAA0.01mg/L的培养基上可以生根. |
| 关 键 词： | 非洲紫罗兰 胚状体 快繁 |
| 收稿时间： | 2004-10-27 |
| 修稿时间： | 2005-09-05 |
Somatic Embryogenic Culture and Rapid Prpagation in vitro of Saintpaulia ionantha from Leaf |
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| SHI Jia-lin,SHI Gui-ying,REN Zhi-bin,HAN Mei-lan,SHI Chun-juan.Somatic Embryogenic Culture and Rapid Prpagation in vitro of Saintpaulia ionantha from Leaf[J].Journal of Plant Genetic Resources,2005,6(4):453-456. | |
| Authors: | SHI Jia-lin SHI Gui-ying REN Zhi-bin HAN Mei-lan SHI Chun-juan |
| Abstract: | Somatic embryoid of Saintpaulia ionantha was obtained from the leaf explants thought rapid propagation.Embryonic cells were preliminary induced on MS+BA0.1mg/L+NAA0.1mg/L+2,4-D1.0mg/L for 15 days,and their regeneration could be increased by 5-10 d initial dark culture.Embryoid could be easily induced on MS+BA0.05-1mg/L.Rapid propagation medium was MS+NAA0.1mg/L +BA0.1 mg/L.Roots could be easily induced on 1/2 MS+NAA0.01mg/L. |
| Keywords: | Saintpaulia ionantha Embryoid Rapid propagation |
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