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利用SSR分子标记检测黄淮夏大豆(Glycine max)初选核心样本的代表性
引用本文:崔艳华,邱丽娟,常汝镇,吕文河.利用SSR分子标记检测黄淮夏大豆(Glycine max)初选核心样本的代表性[J].植物遗传资源学报,2003,4(1):9-15.
作者姓名:崔艳华  邱丽娟  常汝镇  吕文河
作者单位:1. 中国农业科学院作物品种资源研究所,农业部作物种质资源与生物技术重点开放实验室,北京,100081;东北农业大学农学院,哈尔滨,150030
2. 中国农业科学院作物品种资源研究所,农业部作物种质资源与生物技术重点开放实验室,北京,100081
3. 东北农业大学农学院,哈尔滨,150030
基金项目:国家“973”计划资助项目(G1998010203)
摘    要:作物核心种质是用最小的样本代表其全部遗传资源的最大遗传多样性。为了检测大豆初选核心样本取样的代表性,本研究从黄淮夏大豆初选核心样本中,随机选取两个类群,其材料数分别为20份和14份;从保留种质的相应奥群中,分别随机选取6份和5份,共计45份材料,进行14个农艺性状和20对SSR引物的分析。对两组材料进行农艺性状聚类.保菌种质与初选核心种质聚在了一起;利用SSR分子标记数据聚类,也得到了相同的结果。初选核心样本两个类群材料的等位变异数分别为129个,136个;保留种质相应类群材料的等位变异分别为76个,71个;初选核心种质两个类群材料分别包合了整个资源86.00%和86.62%的遗传多样性。本研究为大豆核心种质构建及检测提供分子水平的依据。

关 键 词:核心种质  保留种质  黄淮夏大豆  遗传多样性  SSR分子标记  遗传资源
修稿时间:2002年10月15

Examination of Representiveness of the Primary Core Collection in Huanghuai Summer Sowing Soybean ( Glycine max ) Using SSR
CUI Yan-hu,QIU Li-juan,CHANG Ru-zhen,LU Wen-he.Examination of Representiveness of the Primary Core Collection in Huanghuai Summer Sowing Soybean ( Glycine max ) Using SSR[J].Journal of Plant Genetic Resources,2003,4(1):9-15.
Authors:CUI Yan-hu  QIU Li-juan  CHANG Ru-zhen  LU Wen-he
Abstract:Core collection represents a maximum of the genetic diversity of the whole collection with a minimum of accessions and a minimum of redundancy. In order to test sampling of core collection, the study chose two groups at random among the core collection in Huanghuai summer sowing soybean, with 20 and 14 accessions , respectively; and selected 6 and 5 accessions respectively among the two groups of the reserve collection in Huanghuai summer sowing soybean, torally 45 materials. The study made use of 14 agronomic characters and 20 pairs of SSR primers. The core collection and the reserve collection were clustered together. The two groups in the core collection had 129 and 136 alleles, and the two groups in reserve collection had 76 and 71 alleles. The core collection add up to 86.00% and 86.62% of the diversity of the whole collection. The result indicates that the core collection include genetic diversity of the reserve collection at 70% level. The study provides the proof at molecular level for the evaluation of the availability of the core collection in soybean.
Keywords:Core collection  Reserve collection  Huanghuai summer sowing soybean  Genetic diversity  SSR  
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