应用SRAP标记分析金丝瓜（Cucubitapepo L.）种质资源遗传多样性与亲缘关系

本研究应用SRAP分子标记技术对56份金丝瓜和32份南瓜属（包括西葫芦美洲南瓜、中国南瓜、印度南瓜）种质资源遗传多样性与亲缘关系进行分析。结果表明：（1）从285对SRAP引物中共筛选出50对多态性引物对供试品种基因组DNA进行PCR扩增，获得713条清晰可辨的标记，其中多态性条带634条，多态性比率达88.92%，说明出南瓜属种间遗传多样性较丰富；（2）在56份金丝瓜品种中，共扩增出586条清晰可辨条带，多态性条带350条，多态性比率为59.73%，说明金丝瓜品种间的遗传相似性较高；（3）通过分子聚类分析可将供试金丝瓜品种划分为5个亚类群。金丝瓜与西葫芦美洲南瓜的亲缘关系较近，与中国南瓜的亲缘关系较远，而与印度南瓜的亲缘关系最远；根据分子聚类分析结果证实初步鉴定应可以明确将金丝瓜划归为南瓜属中西葫芦美洲南瓜的一个变亚种。

Genetic Diversity Analysis of Spaghetti Squash and Genetic Relationship to Cucurbita Genus by Using SRAP marker

Abstract: In this study, the SRAP markers were employed to analyze the genetic diversity and relationship between 56 spaghetti squash and 32 Cucurbita genus cultivated species (Including Cucurbita moschata, C. pepo and C.maxima). The results showed that: (1) There were 50 SRAP primer pairs selected from 285 primer pairs, which amplified a total of 713 bands via PCR. Amongst them, 586 bands were polymorphic with a percentage of 88.92%, which suggested that Cucurbita genus was rich in genetic diversity. (2) There were 586 bands generated within the 56 spaghetti squash accessions. 350 (59.7%) of these bands were polymorphic, which revealed that the genetic similarity of spaghetti squash cultivars was high. (3) the 56 spaghetti squash accessions could be clustered into five major sub-groups, according to the analysis on genetic similarity coefficient. Spagetti squash had the closest genetic relationship to C.Pepo; a closer relationship to Cucurbita moschata whearas its relationship with C. Maxima was farthest. According to the above results, we could confirm that the spaghetti squash could be regarded as a variationsubspecies of C. pepo.