| 蚕豆种子贮藏蛋白质组分的比较研究 |
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| 引用本文： | 石建斌,侯万伟,刘玉皎.蚕豆种子贮藏蛋白质组分的比较研究[J].植物遗传资源学报,2012,13(2):304-307. |
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| 作者姓名： | 石建斌 侯万伟 刘玉皎 |
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| 作者单位： | 1. 青海大学,西宁,810016
2. 青海省农林科学院,西宁810016;青海省高原作物种质资源创新与利用-国家重点实验室培育基地,西宁810016 |
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| 基金项目： | 国家973计划前期专项（2010CB134408）,现代农业产业技术体系建设专项（CARS－09）,青海省高校“135高层次人才培养工程”项目 |
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| 摘 要： | 以10份蚕豆品种为材料,利用SDS-PAGE方法,对其种子贮藏蛋白亚基组成进行了分析,结果表明:不同品种间的种子贮藏蛋白具有一定的差异,表现出一定的多态性;共分离出23条迁移率不同的亚基条带,13条具有多态性;利用贮藏蛋白亚基条带的信息,分析了品种间的蛋白相似度,相似度指数0.619～0.947,平均0.744;并通过聚类分析,在遗传相似系数0.8260水平上,将供试材料分为了3类,其中第2类具有较丰富的多样性,多样性指数达3.9296;共筛选出7对亲缘关系较远的杂交组合,可为蚕豆品种选育提供一定的科学依据。
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| 关 键 词： | 蚕豆 种子贮藏蛋白 SDS-PAGE 亲缘关系 蛋白质亚基 |
| 收稿时间： | 2011/12/16 0:00:00 |
| 修稿时间： | 2011/12/16 0:00:00 |
Comparative study on seed storage protein of Faba bean |
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| shijianbin.Comparative study on seed storage protein of Faba bean[J].Journal of Plant Genetic Resources,2012,13(2):304-307. |
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| Authors: | shijianbin |
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| Institution: | 2,3(1Qinghai University,Xining 810016;2Qinghai Academy of Agricultural and Forestry Sciences,Xining 810016;3The Cultivating Base of National Key Lab-Plateau Crop Gene Innovated and Utilized in Qinghai Province,Xining 810016) |
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| Abstract: | In order to investigate the difference of seed storage proteins in Faba bean, Seed storage proteins extracted from ten species of Faba bean were analyzed with SDS-PAGE, the result showed that it is different among the seed storage proteins of faba bean, a total of 23 subunits of different Rf were detected, counting 13 polymorphic protein subunits. The similarity index of seed storage proteins subunits in Faba bean is 0.619~0.947, with an average of 0.744. Cluster analysis divided the accessions into three major clusters in the leave of 0.8260 GS, the second cluster showed higher polymorphism, and 7 pair Faba bean were identified as the parents. |
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| Keywords: | Faba bean Seed storage protein SDS-PAGE Genetic relationship Protein subunits |
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