水稻穗型突变体cl的鉴定、保护与遗传分析

通过神州八号飞船搭载广东省常规优质稻主导品种粤农丝苗获得一个稳定遗传的穗型突变体cl,中文名为粤花占1号,2015年完成农业部植物新品种权的申请工作(申请号:20151728.8,公告号:CNA014491E)。该突变体农艺性状与野生型极为相似,熟色好,产量高,但表现出异常的花序结构,一次枝梗数增加,二次枝梗、小穗梗长度严重缩短,枝梗顶端小穗3粒簇生在一起。进一步的细胞学观测发现,差异主要源自幼穗分化的第3期到第4期发育异常所致。遗传分析表明该性状由非完全显性单基因控制,BSA性状定位法初步将该基因定位于染色体6,命名为CL-6。本研究的结果为进一步精细定位和克隆CL-6基因、找到控制水稻幼穗枝梗伸长缩短的功能基因或调控因子奠定基础,同时为培育理想穗型水稻和观赏农业提供优良种质。

Observation and genetic analysis on rice panicle architecture mutant cl

Abstract: Through the Shen zhou No. 8 spacecraft carrying a good quality rice varieties of Guangdong Province Yue nong si miao, we obtained a stable spike-type mutant cl, named Yue Hua Zhan 1 Hao, which was applied for new plant species rights of the Ministry of Agriculture in 2015( Application number: 20151728.8; Bulletin number: CNA014491E). The mutant had similar agronomic characters with wild type, but abnormal inflorescence structure, with increased primary branches number, seriously shortened secondary branches and spikelets, clustered 3 spikelets on the branch top.Futher cytological observations suggested that the differences were mainly from the third stage to the fourth stage development of young spike differentiation. Genetic analysis showed that the trait was controlled by a pair of semi-dominant gene, and the gene was initially located on chromosome 6, named CL-6(clustered spikelets). The results of this study were lay the foundation for further fine positioning and cloning CL-6 gene, and reveal the functional gene or regulatory factor of rice spikelet elongation and shorten , at the same time offering excellent germplasm to cultivate the ideal panicle rice and ornamental agriculture.