基于SRAP和TRAP标记的河北野生狗牙根种质遗传多样性分析

以SRAP和TRAP 2种标记技术对36份狗牙根材料的遗传多样性及亲缘关系进行了分析,其中包含34份河北省野生狗牙根种质资源。分别由238对SRAP和85对TRAP引物组合中筛选获得具有多态性的SRAP和TRAP引物组合各10对,PCR扩增总条带分别为186和161条,多态性条带156和132条,平均每对引物扩增出多态性条带各15.6和13.2条,多态性位点比率分别为83.4%和81.0%。2种标记合并进行聚类分析,所有供试的36份狗牙根材料遗传相似系数GS=0.519~0.983,平均为0.7。当GS=0.68时,可将36份供试材料分为4个类群。本研究结果表明河北野生狗牙根种质资源存在较丰富的遗传多样性,可为种质资源保护和选育优良狗牙根新品种提供科学依据。

Genetic diversity analysis of wild bermudagrass (Cynodon dactylon) using SRAP and TRAP markers in Hebei Province

SRAP and TRAP markers were employed to investigate the genetic diversity and relationships of 36 bermudagrass (Cynodon dactylon) accessions, including 34 wild bermudagrass germplasm resources of Hebei Province. Ten primer pairs from 238 primers pairs of SRAP and ten primer pairs from 85 primers pairs of TRAP were selected and used for the present study. Ten primers pairs of SRAP and TRAP produced a total of 186 and 161 bands, including 156 and 132 polymorphic bands, averaged 15.6 and 13.2 polymorphic bands per primer pair, and the percentages of polymorphic sites were 83.4% and 81.0% in average, respectively. Combined the both markers with cluster analysis, the genetic similarity among all accessions ranged from 0.519 to 0.983 and was 0.7 in average. When the GS is 0.68, the 36 accessions were classified into four distinct clusters. The results indicated there was substantial genetic diversity among the Cynodon dactylon germplasms in Hebei province, provide a scientific basis for germplasm resources conservation and breeding fine cultivars of bermudagrass.