基于SSR标记的越橘亲缘关系分析及品种鉴定

利用SSR标记对22个越橘栽培品种进行遗传差异及亲缘关系分析,并建立一套稳定的越橘品种鉴定体系,以期为越橘种质资源评价、鉴定、管理及越橘新品种培育奠定基础。本试验优化了一套越橘SSR-PCR反应体系,筛选出15个条带清晰、重复性好、多态性高的SSR标记,对22个越橘栽培品种进行亲缘关系分析,聚类结果与各品种的遗传背景以及表型呈现高度的一致性。从以上15个SSR标记中筛选出可用于越橘品种鉴定的SSR核心引物NA961和NA1040,核心引物组合能够完全区分22个越橘品种。用核心引物制作了参照分子量标记,构建了22个越橘品种的指纹图谱,建立了一套完整的越橘品种鉴定体系。实践验证SSR标记用于越橘品种鉴别的方法可行、结果可靠。

Genetic Relationship Analysis and Cultivar Identification of Blueberry Based on SSR Markers

In this study, we aimed to analyze the genetic relationship of 22 blueberry cultivars and establish a stable and reliable blueberry cultivar identification protocol, which would benefit blueberry germplasm evaluation, identification and management as well as novel variety breeding. We optimized the SSR-PCR reaction system, screened 15 SSR markers producing unambiguous, reproductive and abundant polymorphic bands. A dendrogram of 22 blueberry cultivars was constructed using these 15 SSR markers. It showed great consistency of the dendrogram and genetic background of the analyzed blueberry cultivars. SSR markers NA961 and NA1040 were chosen as core markers from the 15 SSR markers for blueberry cultivar identification, which together could completely distinguish the 22 cultivars. Reference markers and fingerprinting were made using these two core markers. A convenient and reliable blueberry cultivar identification protocol by SSR markers was successfully established