基因枪转化小麦主要影响因素细述

基因枪转化是目前小麦遗传转化的主要技术之一,高效的基因枪转化系统对于转基因小麦新品种培育、候选基因功能鉴定和功能基因组学研究具有重要意义。本文综述了影响基因枪转化小麦效率的主要因素,包括基因型、外植体、植物生长调节剂、轰击参数、筛选体系等,以期为进一步改进小麦基因枪转化技术,提高基因枪转化小麦的效率提供参考。

Main factors influencing the efficiency of biolistic mediated wheat transformation

Physical biolistic particle approach is one of the main techniques used for wheat genetic transformation. Efficient biolistic transformation system is benefit to the development of new wheat varieties, identification of candidate genes, and functional genomics study. In this paper, the main factors including genotypes and explants of wheat, plant growth regulators, bombardment parameters, selection system, etc, which affect wheat transformation efficiency mediated by biolistic bombardment, were reviewed, and the purpose is to provide some useful information for improving the protocol and efficiency of biolistic particle mediated transformation in wheat.