葛根资源遗传多样性和性状关联分析

通过研究葛根资源的遗传多样性和葛根表型性状与分子标记的关联分析,为葛根的分子育种和指纹图谱构建提供理论依据。鉴定分析了127份不同来源葛根资源的10个表型性状,并用ISSR标记研究127份葛根资源的遗传多样性,对ISSR标记与表型性状进行关联分析。表型性状鉴定结果显示葛根资源的10个性状变异大、多样性较好。利用ISSR标记获得多态性条带109条,平均每个引物扩增5.73条、平均Nei's基因多样性0.2085、平均Shannon's指数0.3378,最远遗传距离为0.46。ISSR分子标记聚类分析将127份资源聚为两大类,群体结构分析和PCo A分析结果类似将127份资源分为2个亚群。GLM分析发现3个与茸毛性状关联标记,MLM分析未发现与表型性状关联的标记。本研究收集的资源遗传多样性较好,葛根分子标记聚类结果与地域关系不大,综合GLM和MLM关联分析结果,本试验未发现与表型关联位点。

Genetic Diversity and Trait Association Analysis of Pueraria lobata Resources

Genetic diversity and association analysis of Pueraria lobata (willd.)Ohwi resources are very useful to Pueraria lobata (willd.)Ohwi molecular breeding and fingerprinting construction.In this study,we carried out the genetic diversity and association analysis of 127 resources by using ten botanical characteristics and ISSR markers.The phenotyping results showed that Pueraria lobata(willd.)Ohwi had rich morphological diversity and all the ten botanical characteristics had great variances.109 polymorphic bands were amplified by 19 ISSR primers with an average of 5.73 loci per primer,the mean of Nei's gene diversity was 0.2085,Shannon's information index was 0.3378,and the most distant GD was 0.46.The 127 resources were divided in two main groups by cluster analysis.PCoA and STRUCTURE analysis result corresponding to the analysis by NTSYS,also divided 127 resources into two subgroups,there were three association signals by GLM association analysis and no association signals by MLM association analysis.The high level of genetic diversity was found in our resources,clustering analysis showed that there was little correlation between ISSR marker and geographical distribution,by comparing GLM and MLM association analysis result there wasn't found any association signals.