空心莲子草DNA溶液浸胚处理水稻的变异系分子检测及耐旱性评价

通过SSR标记检测了以空心莲子草DNA溶液浸胚处理获得的10个农艺性状稳定遗传的水稻变异系,结果表明,10个水稻变异系均整合了供体空心莲子草DNA的部分片段。在此基础上,以8个水稻变异系及2个对照为试验材料,采用二因素裂区设计,并以主成分分析、逐步回归分析等多种统计方法分析了变异系的耐旱性。结果表明,以综合评价指标与耐旱指数相结合的复合评价体系,可增强水稻耐旱评价的可靠性。导入系H8最耐旱,H6和H7较耐旱,均优于巴西陆稻。本研究结果对水稻的耐旱性评价与耐旱品种选育具有重要意义。

Molecular Detection of Rice Variant Lines Treated by Embryo-soaking of Alternanthera philoxeroides DNA Solution and Their Drought Resistance Evaluation

Molecular detection was performed by SSR technique for 10 rice variant lines treated by embryo-soaking of Alternanthera philoxeroides DNA solution. The result showed that partial DNA segments of Alternanthera philoxeroides have been transferred into 10 rice variant lines. Based on the molecular evidence, Drought resistance evaluation were further studied using 8 rice variant lines and 2 control varieties in the study, designed by split-plot and analyzed by many statistic methods such as principal component analysis and regression analysis step by step. The results showed that combination the comprehensive evaluation index with drought resistance index could improve the reliability of evaluation for drought tolerance in rice. Cluster analysis based on the two indexes indicated that variant line H8 was the most drought-resistant and H6 and H7 were more drought-resistant than IAPAR9. These results will be important for breeding drought-resistant rice varieties and evaluating drought resistance.