褐色种皮大豆与其黄色种皮衍生亲本的表型及基因型比较

大豆种皮色在从野生大豆到栽培大豆的选择过程中逐渐由黑色变成黄色,是重要的形态标记,因此,大豆种皮色相关基因的研究无论是对进化理论研究还是育种实践都具有非常重要的意义。利用褐色种皮J1265-2大豆及其衍生亲本黄色种皮大豆J1265-1为材料,通过SSR引物扩增片段,检验遗传背景的异同,同时对控制种皮的候选基因GmF3’H进行扩增和测序分析。结果表明,褐色种皮和黄色种皮材料不仅用161对SSR分子标记检测没有发现差异,其褐色种皮候选基因GmF3’H的编码区及起始密码子上游1465 bp序列也是一致的。因此,证明褐色种皮J1265-2大豆与其衍生亲本黄色种皮大豆J1265-1为近等基因系,其控制褐色种皮的基因型与已报道的基因型不同。

Comparative study of phenotype and genotype in soybean and its derivative line with different seed coat colors

Seed coat color, one of the most important morphological markers, is changed from black to yellow during the artificial selection of cultivated soybean from wild soybean. Therefore, discovering genes related to the seed coat color is very important not only for evolutionary study but also for breeding practice in soybean. In this study, Jin1265-1 (yellow seed coat) and its derived line J1265-2 with brown seed coat were used to check whether they had the same genetic background using 161 SSR markers. Meanwhile, The coding sequence and promoter region of a candidate gene (GmF3'H) which was related to seed coat color in soybean was also amplified and sequenced. SSR marker analysis revealed that two lines with different seed coat color had similar genetic background and they were near isogenic lines. There were no sequence difference between two lines in coding region and 1465bp promoter region of GmF3'H , suggesting that gene controlling brown seed coat in J1265-2 was different from the known allele.