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油莎豆SRAP指纹图谱构建及遗传多样性分析
引用本文:赵永国.油莎豆SRAP指纹图谱构建及遗传多样性分析[J].植物遗传资源学报,2013,14(2):222-225.
作者姓名:赵永国
作者单位:中国农业科学院油料作物研究所,农业部油料作物生物学与遗传育种重点实验室,武汉430062
基金项目:国家自然科学基金项目(面上项目,重点项目,重大项目)
摘    要:利用SRAP分子标记构建了14份不同地理来源、表型具有差异的油莎豆品系的分子指纹图谱并进行遗传多样性分析。结果表明100对引物中共有多态性引物42对,扩增出多态性带328条,平均每对引物7.8条。28对引物在12个品系上具有特征谱带,除品系4和14外,均可用1对引物进行鉴定;采用引物组合法仅用Me2/Em6和Me8/Em11这2对引物就可将14份材料区分开,并利用这2对引物构建了上述品系的数字指纹图谱。UPGMA聚类分析表明,所有参试材料间的遗传距离在0.12~0.75之间,平均为0.42,表明我国不同地理来源的油莎豆品系遗传差异较大,具有较为丰富的遗传多样性。

关 键 词:油莎豆  SRAP标记  指纹图谱  遗传多样性
收稿时间:3/1/2012 12:00:00 AM
修稿时间:2012/10/26 0:00:00

Construction of DNA Fingerprinting and Analysis of Genetic diversity with SRAP markers for tigernut (Cyperus esculentus)
Zhao Yong-guo.Construction of DNA Fingerprinting and Analysis of Genetic diversity with SRAP markers for tigernut (Cyperus esculentus)[J].Journal of Plant Genetic Resources,2013,14(2):222-225.
Authors:Zhao Yong-guo
Institution:(Oil Crops Research Institute of Chinese Academy of Agricultural Sciences/Key Laboratory of Biology and Genetic Improvement of Oil Crops,Ministry of Agriculture,Wuhan 430062)
Abstract:The fingerprinting and genetic diversity of 14 tigernut accessions collected from different geographical regions were investigated with SRAP molecular markers. The results showed that 306 polymorphic bands were detected among the tested accessions, with an average of 8.1 bands per primer. Twelve accessions had unique bands by 28 primer pairs, which could be identified with one pair of specific primer expect No 4 and No 14. All 14 accessions could be distinguished by two primer combinations (Me2/Em6 and Me8/Em11) at least, and digital fingerprinting code was also established. Clustering with UPGMA method revealed that the genetic distance ranged from 0.09 to 0.72 with an average of 0.37, which indicated that there was abundant genetic diversity among all 14 accessions.
Keywords:Tigernut  SRAP marker  Fingerprinting  Genetic diversity
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