宁夏60份粳稻种质资源遗传多样性分析

试验用SSR分子标记对60份宁夏粳稻种质资源进行遗传多样性分析。103对SSR引物表现多态性的有58对,共扩增出212条多态性条带,等位变异范围为2～9,平均每对引物3.7个;多态性信息含量(PIC)变幅为0.032～0.788,平均为0.403;高多态性位点主要发生在3号、6号和11号染色体上,而无多态性或低多态性位点主要发生在1号和10号染色体上;成对供试材料的遗传相似系数GS值变幅为0.642～0.958,平均为0.790,单个供试材料的平均GS值变幅为0.710～0.816,平均为0.781,亲缘关系较近;UPGMA聚类表明,在遗传相似系数约0.785处,供试材料可被分为11类,大部分材料被聚在一类中。

Genetic Diversity of 60 Japonica Rice Germplasms

This test focuses on the genetic diversity of 60 Japonica rice Germplasms in Ningxia Province. These Japonica rice germplasms were analyzed by using SSR molecular markers. The results indicated that 58 of 103 pairs SSR primers performed polymorphisms, and 212 alleles were detected. Each pair of primer ranged from 2 to 9 alleles, and the average value was 3.7 alleles. Polymorphic information content (PIC) ranged from 0.032 to 0.788, and the average value was 0.403. High-polymorphic loci emerged mainly at chromosome 3, chromosome 6 and chromosome 11, while non-polymorphic loci and low-polymorphic loci emerged at chromosome 1 and chromosome 10. Genetic similarity of paired varieties ranged from 0.642 to 0.958 and mean was 0.790, while that of single varieties ranged from 0.710 to 0.816 and mean was 0.781. Genetic basis was similar and close genetic relationship. UPGMA cluster analysis showed that 60 rice varieties could be clustered into 11 groups and the overwhelming varieties were clustered into the same group.