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西南冬麦区地方品种HMW-GS组成遗传多样性研究
引用本文:朱炎辉,吉万全,王亚娟,陈雪燕.西南冬麦区地方品种HMW-GS组成遗传多样性研究[J].植物遗传资源学报,2007,8(4):401-405.
作者姓名:朱炎辉  吉万全  王亚娟  陈雪燕
作者单位:西北农林科技大学农学院 陕西杨凌712100(朱炎辉,吉万全,王亚娟),陕西理工学院 汉中723001(陈雪燕)
基金项目:西部地区特色植物种质资源数据平台
摘    要:采用十二烷基硫酸钠-聚丙烯酰胺凝胶电泳(SDS-PAGE)对西南冬麦区(云南、贵州、四川)3个省份共计560份小麦地方品种的高分子量谷蛋白亚基(HMW-GS)组成进行了研究。结果表明:Glu-1位点共有22种等位基因,其中Glu-A1位点4种、Glu-B1位点11种、Glu-D1位点7种;亚基null、7 8和2 12在各自位点的频率最高,分别为89.64%、68.21%和96.43%。亚基组成类型共有46种,以null/7 8/2 12和null/7 9/2 12为主,频率分别为50.89%和11.79%。在这些材料中筛选出一些含有1、2*、17 18、14 15、5 10等优质亚基的材料,其中有52份材料含有优质亚基组合。

关 键 词:小麦地方品种  高分子量麦谷蛋白亚基  遗传多样性
收稿时间:2007-08-20
修稿时间:2007-10-08

Genetic Diversity of HMW-GS Composition in Wheat Landraces from Southwestern Winter Wheat Region
ZHU Yan-hui,JI Wan-quan,WANG Ya-juan,CHEN Xue-yan.Genetic Diversity of HMW-GS Composition in Wheat Landraces from Southwestern Winter Wheat Region[J].Journal of Plant Genetic Resources,2007,8(4):401-405.
Authors:ZHU Yan-hui  JI Wan-quan  WANG Ya-juan  CHEN Xue-yan
Abstract:In order to study the genetic diversity of wheat landraces in southwestern winter wheat region and provide basic information for improving wheat quality,560 wheat landraces in southwestern winter wheat region(Yungui plain vice area,Sichuan valley vice area) were analyzed using SDS-PAGE for the allelic variance of high molecular weight glutenin subunits(HMW-GS).The results showed that there were 22 alleles on Glu-1 loci,including 4 alleles on Glu-A1 locus,11 alleles on Glu-B1 locus,7 alleles on Glu-D1 locus.The frequencies of null,7 8 and 2 12 alleles were the highest on the Glu-A1,Glu-B1 and Glu-D1 loci,which were 89.64%,68.21% and 96.43% respectively.There were 46 subunit composition types,and predominant were null/7 8/2 12 and null/7 9/2 12 types with frequency 50.89% and 11.79%.The landraces with elite subunits of 1,2*,17 18,14 15 and 5 10 were selected and 52 landraces contained elite subunit combinations.
Keywords:SDS-PAGE
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