硬肉桃品种群SSR标记的遗传多样性分析

以45个硬肉桃品种为试材,利用25对位于桃遗传参考图谱上8个连锁群的SSR引物进行了硬肉桃种质资源遗传多样性研究。结果表明:25对SSR引物共获得152个扩增位点,其中多态性位点146个,多态性高达96.05%,Nei′s遗传多样性指数(He)为0.2283,Shannon信息指数(H0)为0.3609。长江流域桃区的硬肉桃品种群体具有最高的Nei′s遗传多样性指数(0.2211)和Shannon信息指数(0.3476),其次为华南亚热带桃区和云贵高原桃区,最低的为华北平原桃区。UPGMA聚类分析结果虽然体现出一定的生态区划特征,但不完全与地理起源相吻合,不同生态区的硬肉桃品种存在一定的交叉;长江流域桃区和云贵高原桃区的硬肉桃品种亲缘关系较近,其次为华南亚热带桃区,最远的为华北平原桃区。本研究鉴定结果更倾向于认为长江流域的硬肉桃可能来源于北方硬肉桃群体,还可能来源于云贵高原生态区和华南亚热带生态区的硬肉桃群体。

Analysis of Genetic Diversity and Relationship of Crisp Peach by SSR

45 crisp peach varieties were used as plant material in this paper.A total of 152 alleles were amplified by 25 primer pairs distributed on eight linkage groups on Prunus reference map.146 alleles showed polymorphism,and the percentage of polymorphie bands (PPB) was 96.05%.The Nei's gene diversity index(He) and Shannon's information index(H0)of crisp peach varieties were 0.2283 and 0.3609,respectively.Crisp peach varieties from the area along Yangtzi river had the highest Nei's gene diversity index(0.2211)and Shannon's information index(0.3476),followed by subtropical zones in South China and Yunnan-Kweichow Plateau,the lowest from the North China Plain.Groups cluster analysis based on the UPGMA method indicated that there was some relationship between crisp peach varieties and ecological zones,but not all varieties were in eomformity with geographic origin and some from different ecological zones overlapped.Crisp peach varieties from the Yangtzi River and YunnanKweichow Plateau had closer genetic relationship,followed by subtropical zones of South China,as far as the North China Plain.The results preferred the opinion that the crisp peach varieties from Yangtzi River may not only origin from the north China.but also from the Yunnan-Kweichow Plateau and subtropical zones in South China.