甘蔗种质间亲缘关系及特异标记的RAPD分析

采用RAPD技术分析甘蔗种质问的亲缘关系及特异标记。筛选出25个扩增多态性较强的随机引物，构建了41份甘蔗种质的RAPD指纹图谱，并对RAPD数据进行UPGMA聚类分析。甘蔗栽培品种之间、栽培品种与近缘种之间以及近缘种相互间的遗传相似系数变异范围为0．56～0．92，表明所研究的甘蔗种质之间的亲缘关系较近。此外，发现某些甘蔗亲本种质具有特异RAPD标记带。

RAPD Analysis on the Relationship and Parental Specific Markers among Sugarcane Germplasm

Relationships and parental specific markers among sugarcane germplasm were analyzed with RAPD. 25 arbitrary primers with polymorphism were selected and used to make the RAPD fingerprinting of 41 sugarcane germplasm. The RAPD data were clustered using UPGMA method. It showed that genetic diversity existed among sugarcane cultivars and relative species. But the genetic similarities were high among these sugarcane germplasm, indicating their close relationships. Some specific RAPD markers were amplified from some sugarcane parental species with some certain arbitrary primers. The parental specific RAPD markers could be observed at a higher frequency in their progenies.