肉质色不同萝卜遗传多样性的SSR分子标记分析

利用微卫星(SSR)标记技术,从600对SSR引物中筛选86对扩增条带清晰的引物,检测了来自我国不同地区37个肉质不同颜色萝卜品种的遗传多样性。86对引物共扩增到976个条带,每对引物扩增出2～17个条带,平均为10.7个,其中多态性条带892个,多态性条带比例为91.39%;共检测出753个基因型,每对引物检测2～20个基因型,平均8.7个,其中有效基因型443.99,有效基因型比例为58.96%;Shannon多态性指数变幅为0.44～2.77,平均1.76。当相似系数为0.81时,可将供试萝卜分成3类,第I类包括6份白色肉质萝卜,第II类包括3份红色肉质萝卜和6份白色肉质萝卜,第III类包括22份红色肉质萝卜。各红色肉质萝卜品种间的遗传相似系数有97%大于0.80,而各白色肉质萝卜品种间的遗传相似系数有91%大于0.80。红色肉质萝卜遗传多样性略低于白色肉质萝卜,红色肉质萝卜与白色肉质萝卜间平均相似系数为0.83,说明不同肉色的萝卜间亲缘关系较密切,在分类上红色肉质萝卜可能是白色萝卜的一个变种。

Genetic Diversity of Radish (Raphanus sativus L.) with Different Fleshy Colors Based on SSR Data

Genetic diversity of 37 radishes(Raphanus sativus L.)with different fleshy colors collected from different areas of China was investigated using 86 SSR primers with distinct amplified bands.86 SSR primers from 600 pairs of SSR primers in Brassica produced stable amplified bands and 976 bands were detected among the 37 accessions with 892 polymorphic bands,accounting for 91.39%.The mean number of alleles was 8.7 with a range from 2 to 20.The effective allelic number was 443.99 and accounted for 58.96%.Shannon′s index varied from 0.44 to 2.77 with an average of 1.76.On the basis of the genetic similarity coefficients,clustering analysis separated the 37 accessions into three groups.The cluster analysis showed that the accessions could be classified into three clusters when the genetic similarity was 0.81.Cluster I included six accessions of which are the white fleshy radishes,cluster II consisted of three red fleshy radishes and six white fleshy radishes,and cluster III composed by 22 accessions of which are the radishes with red fleshy.Genetic similarity coefficients of over 0.80 in the red and white fleshy radishes were 97 and 91%,respectively.The results indicated that there was somewhat higher variation in the red than in the white fleshy radishes.The genetic similarity coefficient between the red and white fleshy radishes was 0.83 indicating genetically close relationship in radishes with different fleshy colors.In addition,it was deduced that the former may be a variant of the latter.