山东省无花果种质资源多样性的RAPD分析

利用随机扩增多态性DNA(RAPD)技术,对58份山东省无花果(Ficus caricaL.)资源的多样性进行了评价,经15个随机引物的初步分析表明,其中至少有23个不同的基因型。继而从36个随机引物中筛选出28个多态性好的引物,进一步对这23个不同基因型材料进行RAPD扩增分析,共得到309个清晰稳定的扩增位点,其中多态性位点236个,占76.4%。利用UPGMA法分析发现,这些基因型间的遗传相似系数在0.592~0.960之间,在此基础上,构建了各基因型间的遗传关系树状图。结果表明,山东省内的无花果资源具有较为丰富的遗传多样性。

Genetic Diversity Analysis of Figs (Ficus carica L.) in Shandong Province by RAPD Technique

The genetic diversity of fig(Ficus carica L.) germplasm resources in Shandong province was evaluated by Randomly Amplified Polymorphic DNA(RAPD) technique.The 58 accessions were classified at least as 23 different genotypes by primary analysis with 15 primers.Then 28 out of 36 primers with high polymorphism were selected for RAPD analysis among these genotypes.There were 309 clear and stable alleles amplified,among them 236 were polymorphic ones(76.4%).The genetic similarity coefficients were between 0.592 and 0.960 among these 23 genotypes by UPGMA(un-weighted pair group method with arithmetic) analysis.And based on those data,the dendrogram of these genotypes was constructed.The result revealed the abundant genetic diversity of fig resources in Shandong province.