西南地区乡土杨树遗传变异的SRAP分析

采用SRAP分子标记技术对西南地区11种乡土杨树共333份样本的遗传变异进行分析,7对引物组合共扩增出215条带,其中多态性条带158条,多态性条带百分率为73.49%,表明11种乡土杨树间存在广泛变异。AMOVA分析结果显示,种间遗传变异分量为10.84,占总变异的48.70%,遗传差异达极显著水平(P0.001)。种间的遗传相似系数变幅在0.8199~0.9607之间,平均遗传相似系数为0.8983。聚类结果表明,昌都杨和藏川杨之间的遗传差异最小,大叶杨和三脉青杨之间的遗传差异最大。本研究结果为西南地区乡土杨树基因资源的保护、开发和利用提供了一定的科学理论依据。

SRAP Analysis of Genetic Variation among Native Poplars in Southwest China

333 samples of native poplars among 11 species collected from Southwest China were examined by SRAP (Sequence-related amplified polymorphism) markers to determine the genetic variation. The results showed that 7 selected primer combinations of SRAP generated 215 bands, of which, 158 bands were polymorphic. The percentage of polymorphic bands was 73.49%. All these index indicated that there were considerable genetic variation existed among 11 native poplar species. The variance components among species was 10.84 and it accounted for 48.70% of the total genetic variation by molecular variance analysis (AMOVA) indicating the genetic difference among 11 native poplar species was significant (P<0.001). The genetic similarity coefficients among the tested poplars ranged from 0.8199 to 0.9607, with an average of 0.8983. The cluster analysis showed that there was the smallest genetic difference between Populus qamdoensis and P. szechuanica var. tibetica, whereas the largest genetic difference between P. lasiocarpa and P. trinervis. The results of this research would provide a scientific basis for conservation, development and utilization of the native poplar gene resources in Southwest China.