应用实时荧光定量PCR技术分析玉米水分胁迫诱导基因的表达模式

应用实时荧光定量PCR(real-time quantitative PCR)技术,研究了玉米中10个水分胁迫诱导基因的相对表达量及表达模式。结果表明,在花丝中,除基因Mads和Grp外,其他8个基因均随干旱胁迫程度加重,相对表达强度增加;在幼穗中,除基因Mads外,其他9个基因均随干旱胁迫程度加重,相对表达强度增加。应用实时荧光定量PCR和宏阵列技术的研究表明,在水分胁迫条件下,基因Arf3、Mads、Trx和F15相对表达量较高,可能在玉米应答水分胁迫过程起更重要的作用。

Identifying Expression Profiles of Water Stress Induced Genes in Maize through Real-time Quantitative PCR

Real-time quantitative PCR was used for identification of expression profiles of 10 water stress induced genes from CN165,a drought-tolerant maize inbred line.The results indicated that all the 10 genes except Mads and Grp greatly increased with increasing intensity of drought stress in silks.In ears,9 of 10 genes significantly increased with increasing intensity of drought stress except Mads.The results based on real-time quantitative PCR and macroarray suggested that Arf3,Mads,Trx and F15 were the four important genes which may play vital roles in drought tolerance.