小麦生长素结合基因 TaABP1-D的定位表达及染色体定位

生长素影响了植物生长发育的诸多过程。生长素结合蛋白 ABP1 (auxin binding protein) 作为一种生长素受体，在质膜上生长素诱导的快速反应中起重要作用。小麦中已经克隆得到了TaABP1-D，但其在细胞中的作用位置以及在染色体定位情况仍不明确。本实验利用洋葱表皮细胞瞬时表达系统对小麦生长素结合基因 TaABP1-D进行亚细胞定位，表明TaABP1-D蛋白为膜蛋白，存在于细胞质和细胞膜中；同时利用中国春缺体-四体材料和信息学方法，将TaABP1-D定位在小麦5D染色体长臂的近着丝粒位置上，距两侧EST标记BE490079和BE405060的遗传距离分别为0.51 cM和0.28 cM。

Expressive and chromosomal localization of TaABP1-D

Auxin affects many processes of plant growth and development. As an auxin receptor, Auxin Binding Protein (ABP1) plays an important role in the rapid reactions induced by auxin on the plasma membrane. The ABP1 in common wheat, named TaABP1-D, have beenwas previously cloned, but not been located on theits chromosome map and the distribution in the cell are still not clear. The distribution of ABP1 in the cell is still unclear. In this study, we located the TaABP1-D on the cytoplasm and cell membrane by transiently expressed system in onion epidermal cells, which indicated that TaABP1-D protein is a membrane protein. In addition, using Chinese Spring nullitetrasomic-tetrasomic lines and bioinformatics methods, we located the TaABP1-D in the pericentromeric region of the long arm of wheat chromosome 5D. EST markers BE490079 and BE405060 flanked TaABP1-D by 0.51 and 0.28 cM, respectively.