强休眠玉米种子休眠前后的蛋白差异表达

以强休眠玉米自交系08-641为试验材料,分别对处于休眠状态下的新鲜收获种子和经过10 d后熟作用破除休眠的种子进行了蛋白质组学差异表达分析。结果表明,通过双向电泳技术在3次重复试验下休眠状态的08-641鲜种子蛋白2-DE图谱上共检测到约600个蛋白质点,在经过10 d后熟作用破除休眠的08-641种子蛋白2-DE图谱上共检测到约620个蛋白质点,其中下调表达蛋白质点4个,上调表达蛋白质点4个,新增蛋白质点8个,缺失表达蛋白质点7个。经过质谱鉴定的差异表达蛋白质主要涉及球蛋白、胚胎晚期丰富蛋白、豆球蛋白等贮藏物蛋白质;蛋白酶体、山梨醇脱氢酶等参与物质代谢的蛋白质;热激蛋白等参与蛋白质结构、细胞功能调控的蛋白质。推测08-641种子休眠是由于种子内休眠相关蛋白的过量表达或缺失抑制了种子的正常萌发。

Proteomic Analysis of storage substances during afterripening of dormant seeds with dry ripening process in maize inbred line 08-641

Maize inbred line 08-641 was used as material to analyze the proteome difference during seed dormancy breaking by mean of 2-D electrophoresis method. The results showed that 600 protein spots were detected in fresh dormant seed and 620 protein spots were detected when seed dormancy was broken through 10d after-ripening, respectively. Among the 23 differential expressions Protein spots, there were 4 down-regulated protein spots, 4 up-regulated proteins, 8 protein spots with additional expression, and 7 protein spots with missing expression. The proteins identified by MALDI-TOF-MS were main involved in globulin, late embryogenesis abundant protein, legumin and other storage proteins; proteasome, sorbitol dehydrogenase which involved in metabolism; heat shock protein involved in protein structure and cell function regulation. The seed dormancy of maize inbred line 08-641 was due to lack of expression or over-expression of some dormancy-related proteins.