| 利用栽培稻C0t-1DNA和基因组DNA比较分析斑点野生稻和短药野生稻基因组 |
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| 引用本文： | 董正伟,柳哲胜,王德彬,蓝伟侦,覃瑞.利用栽培稻C0t-1DNA和基因组DNA比较分析斑点野生稻和短药野生稻基因组[J].植物遗传资源学报,2007,8(3):343-346. |
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| 作者姓名： | 董正伟 柳哲胜 王德彬 蓝伟侦 覃瑞 |
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| 作者单位： | 中南民族大学生命科学学院/国家民委生物技术重点实验室 武汉430074(董正伟，王德彬，覃瑞)，中国农业科学院作物科学研究所 北京100081(柳哲胜)，湖州师范学院生命科学学院 湖州313000(蓝伟侦) |
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| 摘 要： | 通过荧光原位杂交(FISH)利用来源于A基因组栽培稻的中高度重复序列C0t-1DNA和基因组DNA作为探针,对栽培稻、斑点野生稻和短药野生稻进行了比较基因组分析。结果发现C0t-1DNA杂交信号主要分布在这3种染色体的着丝粒、近着丝粒和端粒区域,在斑点野生稻染色体上的信号多于短药野生稻,与gDNA作为探针FISH的结果相一致,说明A和B基因组间的亲缘关系明显近于A和F基因组。确定了含有中高度重复序列的C0t-1DNA用于属内种间关系研究的可行性,并根据C0t-1DNA的FISH结果进行了染色体核型分析。
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| 关 键 词： | 荧光原位杂交 斑点野生稻 短药野生稻 |
| 收稿时间： | 2007/6/13 0:00:00 |
| 修稿时间： | 2007-06-132007-08-09 |
Comparative Analysis of O. punctata and O. brachyantha Genome with Rice C0t-1 DNA and Genomic DNA |
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| DONG Zheng-wei,LIU Zhe-sheng,WANG De-bin,LAN Wei-zhen,QIN Rui.Comparative Analysis of O. punctata and O. brachyantha Genome with Rice C0t-1 DNA and Genomic DNA[J].Journal of Plant Genetic Resources,2007,8(3):343-346. |
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| Authors: | DONG Zheng-wei LIU Zhe-sheng WANG De-bin LAN Wei-zhen QIN Rui |
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| Institution: | 1.Key Laboratory of State Ethnic Affairs Commission for Biological Technology, College of Life Sciences,South-Central University for Nationalities, Wuhan 430074 ;2.Institute of Crop Sciences, Chinese Academy of Agricultural Sciences,Beifing 100081 ; 3. College of Life Sciences, Huzhou Teacher College, Huzhou 313000 |
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| Abstract: | The rice C0t-1 DNA and genomic DNA were used as probes to comparatively analyses Oryza.Sativa,O.punctata and O.brachyantha genome by fluorescence in situ hybridization(FISH).The results showed that C0t-1DNA mainly distributed on centromere,acrocentric and telomere,and there were much more signals on chromosomes in O.punctata than that of in O.brachyantha.This was consistent with that of FISH using gDNA as probe,indicating the relationship between genome A and genome B is much closer than that of between genome A and genome F.The feasibility of the investigation of relationships between species in the genus was confirmed by using highly and moderately repetitive DNA sequences,C0t-1DNA.At the same time,karyotype analysis of O.punctata and O.brachyantha were carried out based on C0t-1 DNA hybridizational signal bands. |
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| Keywords: | C0t-1DNA |
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