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| 萝卜种质资源亲缘关系的RAPD分析 | |
| 引用本文： | 孔秋生,李锡香,向长萍,王海平,宋江萍.萝卜种质资源亲缘关系的RAPD分析[J].植物遗传资源学报,2004,5(2):156-160,169. |
| 作者姓名： | 孔秋生 李锡香 向长萍 王海平 宋江萍 |
| 作者单位： | 1. 中国农业科学院蔬菜花卉研究所,北京,100081;华中农业大学园艺林学学院,武汉,430070 2. 中国农业科学院蔬菜花卉研究所,北京,100081 3. 华中农业大学园艺林学学院,武汉,430070 |
| 基金项目： | 国家基础性工作项目，农业部农作物资源保护项目 |
| 摘 要： | 利用筛选出的12个随机引物,对来源于不同国家和地区的有代表性的56份萝卜种质资源遗传多样性进行了RAPD分析,共扩增出109条带,其中多态性带为72条,多态性带百分率为62.9%,种质平均期望杂合度为0.289.系统聚类分析将供试材料分为2大类、9组,主坐标分析将其分为3大类、7组,两种分类方法结果基本一致,后者能更为直观和清楚地反映群体之间的关系. |
| 关 键 词： | 萝卜 种质资源 聚类分析 主坐标分析 |
| 修稿时间： | 2003年11月28 |
Analysis on the phylogenetic relationship of radish(Raphanus sativus L.) germplasm resources with RAPD |
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| KONG Qiu sheng,LI Xi xiang,XIANG Chang ping,WANG Hai ping,SONG Jing ping.Analysis on the phylogenetic relationship of radish(Raphanus sativus L.) germplasm resources with RAPD[J].Journal of Plant Genetic Resources,2004,5(2):156-160,169. | |
| Authors: | KONG Qiu sheng LI Xi xiang XIANG Chang ping WANG Hai ping SONG Jing ping |
| Institution: | KONG Qiu sheng 1,2,LI Xi xiang 1,XIANG Chang ping 2,WANG Hai ping 1,SONG Jing ping 1 |
| Abstract: | The genetic diversity of radish germplasm resources was analyzed with RAPD. A total of 109 distinct bands were detected with 12 informative RAPD primers among 56 radish accessions from different countries or regions. Of them, 72 bands(62.9%) were polymorphic. The average heterozygosity of RAPD loci was 0.289 among 56 accessions. All accessions were clustered into two groups and nine subgroups based on the RAPD data by the method of clustering analysis, and three groups and seven subgroups by the method of principal coordinates analysis. The results from these two methods were similar, but the result from the principal coordinate analysis was more informative. |
| Keywords: | RAPD |
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