不同SSR标记检测技术及其在花生栽培种遗传多样性分析中的应用

以85份花生栽培种为材料,分别应用银染法和荧光检测法检测9对SSR引物的扩增产物。比较结果显示,荧光检测法具有灵敏度高、检测结果准确、效率高等优点。聚类分析表明,银染法与荧光检测法分别能够区分74个、82个花生品种,并分别聚成8个、9个类群;荧光检测法的聚类结果虽然反映的品种间遗传多样性较低,但与品种类型、产地及其亲缘关系相关程度更高,表明荧光检测数据更精确、可靠。遗传多样性分析发现,地方品种的遗传多样性指数最高,其次为多粒型育成品种,表明我国地方品种和多粒型育成品种蕴藏了丰富的优异性状,有利于对其挖掘和利用。

Different SSR Detection Techniques and their Application in Genetic Diversity Analysis of Peanut (Arachis hypogaea L.) Cultivars

In the present study we assessed silver-staining method and fluorescent detection assay using 9 SSRs and 85 peanut cultivars. The results showed fluorescent detection assay was more sensitive, accurate, and efficient than silver-staining method. Cluster analysis indicated fluorescent detection assay was able to separate 82 peanut cultivars into 9 clusters, while silver-staining method separated 74 cultivars into 8 clusters. The clusters from fluorescent detection assay were more closely related to the types, origins and pedigrees of the peanut cultivars. Genetic diversity analysis showed the peanut landraces had the highest genetic diversity index, followed by the Valencia type peanut cultivars, suggesting the landraces and Valencia type cultivars harboring a broad genetic variation.